Seroprevalence of Brucellosis and Q-Fever in Southeast Ethiopian Pastoral Livestock.
Seroprevalence of Brucellosis and Q-Fever in Southeast Ethiopian Pastoral Livestock.
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DOI：
10.4172/2325-9590.1000109
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发表时间：
2013-03-22
期刊：
影响因子：
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通讯作者：
Schelling E
中科院分区：
文献类型：
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作者：
Gumi B;Firdessa R;Yamuah L;Sori T;Tolosa T;Aseffa A;Zinsstag J;Schelling E
为了评估布鲁氏菌和布氏杆菌在埃塞俄比亚东南部牧场牲畜中的血清流行率，对三种牲畜(牛、骆驼和山羊)进行了横断面研究。该研究于2008年7月至2010年8月期间进行，研究对象包括来自选定地区的8个牧民协会(PA)。用孟加拉玫瑰平板试验(RBPT)对862头牛、458头骆驼和510头山羊共1830份动物血清进行布鲁氏菌初筛。对所有RBPT阳性血清和随机抽取的25%阴性血清进一步进行酶联免疫吸附试验。这些动物共有460头(211头牛、102头骆驼和147头山羊)。从1830头动物的血清中随机抽取20%(180头牛、90头骆驼和98头山羊)，用ELISA法进行伯氏囊虫检测。牛、骆驼和山羊布鲁氏菌的血清感染率分别为1.4%(95%可信区间，0.8-2.6)、0.9%(95%可信区间，0.3-2.7)b和9.6%(95%可信区间，5.2-17.1)。山羊和年龄较大的动物感染风险较高(OR=7.3，95%CI，2.8~19.1)和(OR=1.7，95%CI，0.9~2.9)。在98份RBPT阴性的骆驼血清中，酶联免疫吸附试验阳性率为12.0%。牛、骆驼和山羊的伯氏梭菌血清感染率分别为31.6%(95%CI，24.7~39.5)、90.0%(95%CI，81.8~94.7)和54.2%(95%CI，46.1~62.1)。我们在所有动物物种的所有受测PA中发现了伯氏梭菌试验阳性动物。骆驼和年龄较大的动物是感染的危险因素(OR=19.0，95%CI，8.9~41.2)和(OR=3.6，95%CI，2.0~6.6)。在所有被测试的牲畜中，伯氏梭菌的血清阳性率较高，在山羊中布鲁氏菌的血清阳性率较高，这意味着人类感染这两种疾病的风险。因此，有必要进一步研究这两种疾病在该地区的动物和人类。
To assess seroprevalences of Brucella and C. burnetii in pastoral livestock in southeast Ethiopia, a cross-sectional study was carried out in three livestock species (cattle, camels and goats). The study was conducted from July 2008 to August 2010, and eight pastoral associations (PAs) from the selected districts were included in the study. Sera from a total of 1830 animals, comprising 862 cattle, 458 camels and 510 goats were screened initially with Rose Bengal plate test (RBPT) for Brucella. All RBPT positive and 25% of randomly selected negative sera were further tested by ELISA. These comprise a total of 460 animals (211 cattle, 102 camels and 147 goats). Out of sera from total of 1830 animals, 20% were randomly selected (180 cattle, 90 camels and 98 goats) and tested for C. burnetii using ELISA. The seroprevalences of Brucella was 1.4% (95% confidence interval (CI), 0.8-2.6), 0.9% (95% CI, 0.3-2.7)b and 9.6% (95% CI, 5.2-17.1) in cattle, camels and goats, respectively. Goats and older animals were at higher risk of infection (OR=7.3, 95% CI, 2.8-19.1) and (OR=1.7 95% CI, 0.9-2.9), respectively. Out of 98 RBPT negative camel sera, 12.0% were positive for ELISA. The seroprevalences of C. burnetii were 31.6% (95% CI, 24.7-39.5), 90.0% (95% CI, 81.8-94.7) and 54.2% (95% CI, 46.1-62.1) in cattle, camels and goats, respectively. We found positive animals for C. burnetii test in all tested PAs for all animal species. Being camel and older animal was a risk factor for infection (OR=19.0, 95% CI, 8.9-41.2) and (OR=3.6, 95% CI, 2.0-6.6), respectively. High seropositivity of C. burnetii in all livestock species tested and higher seropositive in goats for Brucella, implies risks of human infection by both diseases. Thus, merit necessity of further study of both diseases in animals and humans in the area.