PURIFICATION OF HUMAN PLATELET-DERIVED GROWTH-FACTOR
PURIFICATION OF HUMAN PLATELET-DERIVED GROWTH-FACTOR
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DOI：
10.1073/pnas.76.4.1809
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
STILES, CD
中科院分区：
文献类型：
--
作者：
ANTONIADES, HN;SCHER, CD;STILES, CD
人血小板含有刺激结缔组织细胞增殖的多肽生长因子。通过加热（100 ° C）完成该血小板衍生生长因子（PDGF）的纯化C）处理洗涤的血小板，随后进行离子交换层析，在1 M乙酸中凝胶过滤，等电聚焦和制备型十二烷基硫酸钠/聚丙烯酰胺凝胶电泳。PDGF的等电点为9.8，MW范围为13，000 - 16，000，通过在1 M乙酸中的凝胶过滤或在还原条件下的分析型十二烷基硫酸钠凝胶电泳判断。纯化的PDGF的比活性比在未分级分离的人血清中发现的高2000万倍。纯化的PDGF在浓度为1 ng/ml（0.1 nM）时刺激BALB/c [大鼠肿瘤成纤维细胞] 3 T3细胞的静态密度抑制培养物中的复制DNA合成和细胞增殖。
Human platelets contain a polypeptide growth factor that stimulates the proliferation of connective tissue cells. Purification of this platelet-derived growth factor (PDGF) was accomplished by heat (100.degree. C) treatment of washed platelets and subsequent ion-exchange chromatography, gel filtration in 1 M acetic acid, isoelectric focusing and preparative sodium dodecyl sulfate/polyacrylamide gel electrophoresis. PDGF had an isoelectric point of 9.8 and a MW ranging from 13,000 to 16,000 as judged by gel filtration in 1 M acetic acid or analytical sodium dodecyl sulfate gel electrophoresis under reducing conditions. The specific activity of the purified PDGF was 20 million times greater than that found in unfractionated human serum. Purified PDGF stimulated replicative DNA synthesis and cell proliferation in quiescent density-arrested cultures of BALB/c [rat neoplastic fibroblasts] 3T3 cells at concentrations of 1 ng/ml (0.1 nM).