Requirement of regulated endocrine-specific protein-18 for development and expression of regulated endocrine-specific protein-18 isoform c in mice
Requirement of regulated endocrine-specific protein-18 for development and expression of regulated endocrine-specific protein-18 isoform c in mice
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DOI：
10.1007/s11033-010-0394-6
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发表时间：
2011-04
影响因子：
2.8
通讯作者：
Minrui Liang;Jian Yang;Min-juan Bian;Jie Liu;Xiaoqi Hong;Yan Wang;Yulu Huang;S. Gu;Mei Yu;Fang Huang;J. Fei
中科院分区：
文献类型：
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作者：
Minrui Liang;Jian Yang;Min-juan Bian;Jie Liu;Xiaoqi Hong;Yan Wang;Yulu Huang;S. Gu;Mei Yu;Fang Huang;J. Fei
调节内分泌特异性蛋白18（Regulated endocrine specific protein-18，R18）主要分布于外周内分泌和神经内分泌组织。β 18蛋白的表达受生理因素如血糖或多巴胺能药物的调节，但其功能尚不清楚。本研究通过建立小鼠E18杂合子缺陷模型，进一步发现E18在胚胎发育过程中起重要作用，以探讨E18在体内的生物学功能。此外，我们还通过逆转录-聚合酶链反应（RT-PCR）克隆了一个新的小鼠β 18亚型，命名为β 18-c。小鼠胸腺素18-c通过跳读外显子4（长度为43 bp），编码120个氨基酸残基的较短蛋白。在小鼠外周组织和脑组织中，α 18-cmRNA的分布与α 18-cmRNA的分布相似。
Regulated endocrine-specific protein-18 (RESP18) is distributed mainly in the peripheral endocrine and neuroendocrine tissues. The expression of RESP18 protein is regulated by physiological factors, such as blood glucose or dopaminergic drugs, but its functions remain unclear. In this study, to explore the biological functions of RESP18 in vivo, we generatedRESP18heterozygous deficient mice, and further found RESP18 was essential for embryonic development. In addition, we cloned a new isoform of mouseRESP18by reverse transcription-polymerase chain reaction (RT-PCR), and denominated it asRESP18-c. MouseRESP18-c, by skipping exon4 (43 bp in length), encodes a shorter protein of 120 amino acid residues. The distribution ofRESP18-cmRNA is similar with that ofRESP18 mRNA in the peripheral tissues and brains of mice.