Genome structure and expression of the ev/J family of avian endogenous viruses.
Genome structure and expression of the ev/J family of avian endogenous viruses.
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禽内源病毒ev/J家族的基因组结构和表达。
DOI：
10.1128/jvi.73.7.5345-5355.1999
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发表时间：
1999
影响因子：
5.4
通讯作者：
Conklin,KF
中科院分区：
文献类型：
--
作者：
Ruis,BL;Benson,SJ;Conklin,KF
我们最近报道了鸡基因组中与J亚群禽白血病病毒（S。J. Benson，B. L. Ruis，A. M.很好，K。F. Conklin，J. Virol. 72：10157-10164，1998）。基于内源性J亚群相关基因与长末端重复序列（LTR）相关的事实，我们得出结论，这些LTR-env序列定义了一个新的禽类内源性病毒家族，我们将其命名为ev/J家族。在这份报告中，我们进一步的EV/J前病毒的内容和表达的特点。所获得的数据表明，有6和11个拷贝之间的ev/J前病毒在所有的鸡细胞检查，这些前病毒分为六类。18个前病毒检查，都有高度的序列同一性，都含有一个内部缺失，删除所有的polgene和各种数量的gagandenvgene序列。几个ev/J前病毒的thegaggenes，LTR和非翻译区的测序揭示了分离株之间的高水平的同一性，表明它们没有经历过显着的序列变异，因为它们引入到禽生殖系。虽然ev/Jgag基因与禽白血病肉瘤病毒家族的关系相对较弱（在氨基酸水平上46%的同一性和61%的相似性），但它保留了几个对病毒组装、出芽和/或感染性具有重要意义的序列。最后，获得的证据表明，至少有一些成员的ev/J家族的表达，如果翻译，可以编码Gag和Env相关的多肽。
We recently reported the identification of sequences in the chicken genome that show over 95% identity to the novel envelope gene of the subgroup J avian leukosis virus (S. J. Benson, B. L. Ruis, A. M. Fadly, and K. F. Conklin, J. Virol. 72:10157–10164, 1998). Based on the fact that the endogenous subgroup J-relatedenvgenes were associated with long terminal repeats (LTRs), we concluded that these LTR-envsequences defined a new family of avian endogenous viruses that we designated the ev/J family. In this report, we have further characterized the content and expression of the ev/J proviruses. The data obtained indicate that there are between 6 and 11 copies of ev/J proviruses in all chicken cells examined and that these proviruses fall into six classes. Of the 18 proviruses examined, all share a high degree of sequence identity and all contain an internal deletion that removes all of thepolgene and various amounts ofgagandenvgene sequences. Sequencing of thegaggenes, LTRs, and untranslated regions of several ev/J proviruses revealed a high level of identity between isolates, indicating that they have not undergone significant sequence variation since their introduction into the avian germ line. Although the ev/Jgaggene showed a relatively weak relationship (46% identity and 61% similarity at the amino acid level) to that of the avian leukosis-sarcoma virus family, it retains several sequences of demonstrated importance for virus assembly, budding, and/or infectivity. Finally, evidence was obtained that at least some members of the ev/J family are expressed and, if translated, could encode Gag- and Env-related polypeptides.