Ski is involved in transcriptional regulation by the repressor and full-length forms of Gli3
Ski is involved in transcriptional regulation by the repressor and full-length forms of Gli3
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DOI：
10.1101/gad.1017302
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发表时间：
2002-11-15
影响因子：
10.5
通讯作者：
Ishii, S
中科院分区：
文献类型：
--
作者：
Dai, P;Shinagawa, T;Ishii, S
转录因子胶质母细胞瘤-3 （Gli3）在肢体芽的前部被切割以产生其抑制形式。相反，来自极化活性后区的Sonic hedgehog （Shh）信号阻断Gli3加工，然后诱导Gli3靶基因的表达，包括Gli1。在这里，我们报道Ski协同抑制因子结合Gli3并招募组蛋白去乙酰化酶复合物。Gli3介导的抑制被抗Ski抗体和Ski缺陷成纤维细胞破坏，全长Gli3介导的sh诱导的Gli1基因转录被Ski抑制。此外，Ski突变增强了Gli3基因突变引起的手指异常。因此，滑雪在图案形成中起着重要的作用。
Transcription factor Glioblastoma-3 (Gli3) is cleaved in the anterior region of the limb bud to generate its repressor form. In contrast, Sonic hedgehog (Shh) signaling from the posterior zone of polarizing activity blocks Gli3 processing and then induces the expression of Gli3 target genes, including Gli1. Here we report that the Ski corepressor binds to Gli3 and recruits the histone deacetylase complex. The Gli3-mediated repression was impaired by anti-Ski antibody and in Ski-deficient fibroblasts, and Shh-induced Gli1 gene transcription mediated by full-length Gli3 was inhibited by Ski. Furthermore, a Ski mutation enhanced the digit abnormalities caused by the Gli3 gene mutation. Thus, Ski plays an important role in pattern formation.