Microscale electrokinetic assessments of proteins employing insulating structures
Microscale electrokinetic assessments of proteins employing insulating structures
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DOI：
10.1016/j.coche.2020.02.007
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发表时间：
2020-09-01
影响因子：
6.6
通讯作者：
Lapizco-Encinas, Blanca H.
中科院分区：
文献类型：
--
作者：
Lapizco-Encinas, Blanca H.
微尺度电动系统为蛋白质的分析提供了诱人的特性；特别是依赖于线性和非线性电动效应的系统已被证明是传感、分选和纯化蛋白质颗粒的有效平台。目前的贡献集中在评估含有用于产生非线性EK场的绝缘结构的装置中的蛋白质样品(蛋白质溶液、外切体、蛋白质纳米晶)。这种装置结合了几种电动现象，使粒子能够进行有效的操纵，特别是在悬浮介质和施加的电位特性经过仔细微调的情况下。对微尺度电动装置中蛋白质操纵的最新发现进行了分析和讨论，突出了几个领先研究小组所获得的有价值的发现。
Microscale electrokinetic systems have offer attractive characteristics for the analysis of proteins; in particular, systems that rely on both, linear and nonlinear electrokinetic effects, have proven to be effective platforms for sensing, sorting and purifying protein particles. The present contribution is focused on the assessment of protein samples (protein solutions, exosomes, protein nanocrystals) in devices that contains insulating structures for the generation of nonlinear EK fields. Such devices combine several electrokinetic phenomena that enable effective particle manipulation, especially when suspending medium and applied potential characteristics are carefully fine-tuned. An analysis of the latest findings on protein manipulation in microscale electrokinetic devices is presented and discussed, highlighting the valuable findings obtained by several leading research groups.