SENSORY PHYSIOLOGY, ANATOMY AND IMMUNOHISTOCHEMISTRY OF ROHON-BEARD NEURONS IN EMBRYOS OF XENOPUS-LAEVIS
SENSORY PHYSIOLOGY, ANATOMY AND IMMUNOHISTOCHEMISTRY OF ROHON-BEARD NEURONS IN EMBRYOS OF XENOPUS-LAEVIS
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DOI：
10.1113/jphysiol.1984.sp015122
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发表时间：
1984-01-01
影响因子：
5.5
通讯作者：
ROBERTS, A
中科院分区：
文献类型：
--
作者：
CLARKE, JDW;HAYES, BP;ROBERTS, A
Rohon-Beard神经元的胞体和中央突出的轴突显示p样物质免疫活性。外周，用辣根过氧化物酶染色显示干皮肤内游离神经末梢的形态。利用细胞内微电极研究了皮肤自然和电刺激对罗洪-比尔德神经元的刺激。罗洪-比尔德细胞对躯干皮肤上短暂的局部压痕很敏感，对一个或几个脉冲作出反应。他们能迅速适应反复的刺激。它们也可以通过短暂的电流脉冲刺激皮肤。它们对皮肤的缓慢压痕不敏感，也不受上皮动作电位的刺激。对皮肤刺激的反应不会被含有12 mM Mg2+和仅0.5 mM Ca2+的林格溶液消除。单个罗洪-比尔德细胞的细胞内诱发动作电位有时足以引起持续的有效游泳。结果表明，罗洪-比尔德细胞负责探测到对胚胎身体的轻触刺激，并根据这种刺激开始游泳。
Rohon-Beard neurons show substance P-like immunoactivity in their somas and in their centrally projecting axons. Peripherally, the morphology of their free nerve endings within the trunk skin has been shown using horseradish peroxidase staining. The excitation of Rohon-Beard neurons by natural and electrical stimulation of the skin has been examined using intracellular micro-electrodes in the late embryo of X. laevis. Rohon-Beard cells are sensitive to transient, local indentation of the trunk skin, responding with 1 or a few impulses. They adapt rapidly to repeated stimulation. They can also be excited by a brief current pulse to the skin. They are not sensitive to slow indentation of the skin, nor are they excited by epithelial action potentials. The responses to skin stimulation are not abolished by a Ringer solution containing 12 mM Mg2+ and only 0.5 mM Ca2+. Intracellularly evoked action potentials in single Rohon-Beard cells are sometimes sufficient to evoke sustained episodes of fictive swimming. The results indicate that Rohon-Beard cells are responsible for detecting light touch stimuli to the embryo''s body and for initiating swimming in response to this stimulus.