Selective targeting of newly synthesized Arc mRNA to active synapses requires NMDA receptor activation
Selective targeting of newly synthesized Arc mRNA to active synapses requires NMDA receptor activation
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DOI：
10.1016/s0896-6273(01)00275-6
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发表时间：
2001-04-01
期刊：
影响因子：
16.2
通讯作者：
Worley, PF
中科院分区：
文献类型：
--
作者：
Steward, O;Worley, PF
新合成的ARE信使核糖核酸选择性地定位于经历了特定活动模式的突触。在这里，我们证明了靶向需要NMDA受体的激活。ARE的表达是由电惊厥诱导的，然后新合成的mRNA通过激活向齿状回的穿支路径投射而被靶向突触位置。在刺激期间，将含有NMDA受体拮抗剂(MK801或APV)的微管电极放置在齿状回，新合成的ARE mRNA被输送到树突，但不定位于激活的板层，而是保持弥漫性分布。AMPA受体拮抗剂(CNQX)阻断ARE mRNA的小区域靶向，mGluR拮抗剂(MCPG)不影响定位。这些结果表明，靶向活性突触的ARE基因需要NMDA受体的激活。
Newly synthesized Are mRNA is selectively targeted to synapses that have experienced particular patterns of activity. Here, we demonstrate that the targeting requires NMDA receptor activation. Are expression was induced by an electroconvulsive seizure, and the newly synthesized mRNA was then targeted to synaptic sites by activating the perforant path projections to the dentate gyrus. When micropipette electrodes containing NMDA receptor antagonists (MK801 or APV) were positioned in the dentate gyrus during the stimulation period, newly synthesized Are mRNA was transported into dendrites but did not localize in the activated lamina; instead, the mRNA remained diffusely distributed. AMPA receptor antagonists (CNQX) blocked targeting of Are mRNA in a small region, and mGluR antagonists (MCPG) did not affect localization. These results demonstrate that NMDA receptor activation is required for the targeting of Are mRNA to active synapses.