COMPARISON OF EFFECTS OF ULTRAVIOLET-LIGHT AND PURINERGIC NERVE-STIMULATION ON GUINEA-PIG TAENIA-COLI
COMPARISON OF EFFECTS OF ULTRAVIOLET-LIGHT AND PURINERGIC NERVE-STIMULATION ON GUINEA-PIG TAENIA-COLI
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DOI：
10.1111/j.1476-5381.1978.tb08459.x
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发表时间：
1978-01-01
影响因子：
7.3
通讯作者：
WONG, H
中科院分区：
文献类型：
--
作者：
BURNSTOCK, G;WONG, H
1豚鼠结肠带、膀胱和兔门静脉对紫外线的反应光与嘌呤能神经刺激和外源性三磷酸腺苷（ATP）引起的光进行比较。2在亚硝酸钠存在的情况下，340 - 380 nm之间的u. v.光使结肠带产生最大松弛。这种松弛是可逆的，而且起效快。阿托品、乙啶或低浓度酚妥拉明或普萘洛尔均不影响该反应。当音调较低时，放松通常伴随着刺激停止后的“反弹收缩”。因此，对紫外光的反应非常类似于对嘌呤能神经刺激和外源性应用ATP的反应。3U.v.光没有在嘌呤能神经中引发冲动，因为它的作用不受河豚毒素的影响;它也没有从神经末梢释放ATP（与其在嘌呤能神经刺激期间的释放相反）。腺苷摄取抑制剂双嘧达莫可增强对嘌呤能神经刺激和ATP的反应，但不影响对紫外光的反应。4已知可改变对嘌呤能神经刺激和ATP的连接后反应的药物也改变了对紫外光的反应。高浓度的2-取代咪唑啉化合物安他唑啉和酚妥拉明可拮抗对嘌呤能神经刺激和ATP的反应，降低对紫外线照射的反应。前列腺素合成抑制剂吲哚美辛可消除刺激嘌呤能神经后的“反弹收缩”，也可阻断紫外线照射后的“反弹收缩”。增加K+浓度可引起对紫外光和嘌呤能神经刺激的抑制反应平行变化，5 U. v光可引起兔门静脉自发活动的舒张和抑制（由ATP放松），但对豚鼠膀胱没有影响（由ATP收缩）和输尿管（不受ATP影响）提示紫外光作用于参与抑制反应介导的嘌呤能受体复合物的某些部分ATP和嘌呤能神经刺激，因此可能提供一种研究抑制性嘌呤能受体化学的方法。
1The responses of the guinea‐pig taenia coli, urinary bladder and the rabbit portal vein to ultraviolet (u.v.) light were compared to those elicited by purinergic nerve stimulation and exogenous adenosine triphosphate (ATP).2In the presence of sodium nitrite, u.v. light between 340‐380 nm produced a maximum relaxation of the taenia coli. The relaxation was reversible and fast in onset. It was unaffected by atropine, guanethidine or low concentrations of phentolamine or propranolol. When the tone was low, the relaxation was usually followed by a ‘rebound contraction’ upon cessation of stimulation. Thus, the response to u.v. light closely resembles the responses to both purinergic nerve stimulation and exogenously applied ATP.3U.v. light did not initiate impulses in purinergic nerves since its action was unaffected by tetrodotoxin; nor did it release ATP from nerve terminals (in contrast to its release during purinergic nerve stimulation). The adenosine‐uptake inhibitor, dipyridamole, which potentiates the responses to purinergic nerve stimulation and ATP, did not affect the response to u.v. light.4Agents known to alter postjunctional responses to purinergic nerve stimulation and ATP also altered the response to u.v. light. High concentrations of the 2‐substituted imidazoline compounds, antazoline and phentolamine, which antagonize the responses to purinergic nerve stimulation and ATP, reduced the responses to u.v. irradiation. The prostaglandin synthesis inhibitor, indomethacin, which abolishes the ‘rebound contraction’ following stimulation of purinergic nerves, also blocks the ‘rebound ‘contraction’ following u.v. irradiation. Increases in the K+concentration produced parallel changes in the inhibitory responses to u.v. light and purinergic nerve stimulation.5U.v. light produced relaxation and inhibition of spontaneous activity of the rabbit portal vein (relaxed by ATP), but had no effect on the guinea‐pig urinary bladder (contracted by ATP) and ureter (unaffected by ATP).6It is suggested that u.v. light is acting on some part of the purinergic receptor complex which is involved in the mediation of inhibitory responses to ATP and purinergic nerve stimulation, and may therefore provide a way of investigating the chemistry of inhibitory purinergic receptors.