Comparative genome analysis of Salmonella Enteritidis PT4 and Salmonella Gallinarum 287/91 provides insights into evolutionary and host adaptation pathways
Comparative genome analysis of Salmonella Enteritidis PT4 and Salmonella Gallinarum 287/91 provides insights into evolutionary and host adaptation pathways
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DOI：
10.1101/gr.077404.108
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发表时间：
2008-10-01
期刊：
影响因子：
7
通讯作者：
Parkhill, Julian
中科院分区：
文献类型：
--
作者：
Thomson, Nicholas R.;Clayton, Debra J.;Parkhill, Julian
我们测定了寄主混杂的肠炎沙门氏菌PT4株P125109和鸡限制性肠炎沙门氏菌287/91株的全基因组序列。这些沙门氏菌与其他沙门氏菌的基因组比较表明，加氏沙门氏菌287/91是肠炎沙门氏菌的新进化后代。值得注意的是，由于缺失和假基因的形成，鸡链霉菌的基因组发生了广泛的降解。将鸡沙门氏菌中的假基因与以前在其他宿主适应细菌中发现的假基因进行比较，揭示了许多常见功能特征的丧失，并为宿主和组织适应的可能机制提供了见解。我们认为，对鸡和小鼠的肠炎链球菌进行的实验分析--存在鸡链球菌假基因同源基因的突变--可以提供一条实验上容易处理的途径，以揭开肠炎链球菌宿主适应的遗传基础。
We have determined the complete genome sequences of a host-promiscuous Salmonella enterica serovar Enteritidis PT4 isolate P125109 and a chicken-restricted Salmonella enterica serovar Gallinarum isolate 287/91. Genome comparisons between these and other Salmonella isolates indicate that S. Gallinarum 287/ 91 is a recently evolved descendent of S. Enteritidis. Significantly, the genome of S. Gallinarum has undergone extensive degradation through deletion and pseudogene formation. Comparison of the pseudogenes in S. Gallinarum with those identified previously in other host-adapted bacteria reveals the loss of many common functional traits and provides insights into possible mechanisms of host and tissue adaptation. We propose that experimental analysis in chickens and mice of S. Enteritidis-harboring mutations in functional homologs of the pseudogenes present in S. Gallinarum could provide an experimentally tractable route toward unraveling the genetic basis of host adaptation in S. enterica.