Three microsatellite multiplex PCR assays allowing high resolution genotyping of white spruce, Picea glauca
Three microsatellite multiplex PCR assays allowing high resolution genotyping of white spruce, Picea glauca
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DOI：
10.1515/sg-2014-0029
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发表时间：
2014-01-01
期刊：
影响因子：
1
通讯作者：
Schnittler, M.
中科院分区：
文献类型：
--
作者：
Eusemann, P.;Herzig, P.;Schnittler, M.
15个以前发表的微卫星引物对加拿大白色云杉的扩增和多态性进行了筛选，并测试其在PCR多重适用性。11个基因座表达的多态性范围从7到58个等位基因被选中的开发和优化的三个多重检测。使用总共包含1470棵树的四个自然林分来表征所选基因座，并证明它们对于基因分型研究和亲子关系分析的适用性。这些分析可用于研究重点群体遗传学，亲子关系分析，种源研究，或个人遗传指纹。多重PCR的使用通过同时实现高分辨率并减少处理时间和每个样品的成本来促进大规模研究。
Fifteen previously published microsatellite primer pairs developed for and tested on Canadian white spruce were screened for amplification and polymorphy in Alaskan populations and tested for their suitability in PCR multiplexing. Eleven loci expressing polymorphisms ranging from 7 to 58 alleles were selected for development and optimization of three multiplex assays. Four natural stands containing a total of 1470 trees were used to characterize the selected loci and demonstrate their applicability for genotyping studies and parentage analysis. These assays can be used for studies focusing on population genetics, parentage analysis, provenance research, or individual genetic fingerprinting. The use of multiplex PCR facilitates large-scale studies by simultaneously enabling high resolution and reducing processing time and per sample cost.