Tamoxifen-induced DNA adducts in leucocytes of breast cancer patients
Tamoxifen-induced DNA adducts in leucocytes of breast cancer patients
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DOI：
10.1093/carcin/18.1.9
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发表时间：
1997-01-01
期刊：
影响因子：
4.7
通讯作者：
Hansson, J
中科院分区：
文献类型：
--
作者：
Hemminki, K;Rajaniemi, H;Hansson, J
应用P-32后标记法，以大鼠肝DNA为阳性标准，对乳腺癌患者白细胞DNA进行检测，发现三苯氧胺（Tamoxifen）诱导的DNA加合物，并对治疗组和对照组的总白色血细胞DNA进行了分析。五个对照中没有一个是阳性的。通过使用不同的色谱系统，每一个都使用掺加的大鼠肝脏样品，证实了作为他莫昔芬加合物的级分的身份。治疗患者的加合物水平为5.5个加合物/10（9）个核苷酸，而对照组的表观水平为1.9/10（9）。
Tamoxifen-induced DNA adducts were searched in leucocyte DNA from breast cancer patients, Total white blood cell DNA from tamoxifen-treated and control patients was analysed by P-32-postlabelling using HPLC-radioactivity detection, Rat liver DNA was used as a positive standard, In blinded analysis four of the six treated patients showed DNA adducts; none of the five controls mere positive, The identity of fraction as a tamoxifen adduct was confirmed by using different chromatographic systems, each,vith spiked rat liver samples. The level of adducts in the treated patients was 5.5 adducts/10(9) nucleotides as compared to an apparent level of 1.9/10(9) in the controls.