Increase of gingival matured dendritic cells number in elderly patients with chronic periodontitis
Increase of gingival matured dendritic cells number in elderly patients with chronic periodontitis
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DOI：
10.1016/j.archoralbio.2008.06.014
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发表时间：
2009-01-01
影响因子：
3
通讯作者：
Seguier, Sylvie
中科院分区：
文献类型：
--
作者：
Bodineau, Agnes;Coulomb, Bernard;Seguier, Sylvie
目的：本研究的目的是评价老年和年轻泛发性慢性牙周炎患者上牙龈结缔组织中包括成熟树突状细胞（DC）在内的炎症细胞亚群比例，以进一步了解年龄对牙龈炎症现象的影响。8例75岁以上老年人慢性牙周炎牙龈组织标本使用抗CD 45 RB、CD 4、CD 8、CD 19、CD 68、DC-SIGN、DC-LAMP分子的单克隆抗体，通过免疫组织化学分析来自8名年龄在50-60岁的年轻患者（被认为是对照，C组）的患者（试验组，T组）和来自8名年龄在50-60岁的年轻患者（被认为是对照，C组）。结果：各组间上牙龈结缔组织中CD 45 RB+白细胞数量差异不显著，可作为对照。相比之下。T组淋巴细胞亚群/CD 45 RB+细胞比值较C组有下降趋势，但仅CD 4 + T淋巴细胞/CD 45 RB+细胞比值下降有显著性意义（p < 0.03）。相反，抗原呈递细胞DC-SIGN +细胞/CD 45 RB+细胞和DC-LAMP +细胞/CD 45 RB+细胞的比率显著增加;（p < 0.03和p <0.05）。
Objective: The purpose of this study was to evaluate the inflammatory cell subset proportions in the upper gingival connective tissue, including mature dendritic cells (DC) in elderly and younger patients with generalized chronic periodontitis in order to further understand the effect of aging on gingival inflammatory phenomenon.Methods: Gingival tissue specimens presenting chronic periodontitis from 8 elderly patients aged >75 (test group, group T) and from 8 younger patients aged 50-60 (considered as controls, group C) were analysed by immunohistochemistry using monoclonal antibodies against CD45RB, CD4, CD8, CD19, CD68, DC-SIGN, DC-LAMP molecules. The number of each immunolabelled cells subset was counted using image analysis.Results: The difference in the number of CD45RB + leucocytes in the upper gingival connective tissue between groups was not significant permitting to use it as reference. As compared. to group C, the lymphocyte subsets/CD45RB + leucocytes ratios tended to decrease in group T but the decrease was significant only for CD4 + T lymphocytes/ CD45RB + cells ratio (p < 0.03). On the opposite, the ratios of antigen-presenting cells DC-SIGN + cells/CD45RB + cells and DC-LAMP + cells/CD45RB + cells were significantly increased;(p < 0.03 and