Ca(2+) entry through store-operated channels in mouse sperm is initiated by egg ZP3 and drives the acrosome reaction.
Ca(2+) entry through store-operated channels in mouse sperm is initiated by egg ZP3 and drives the acrosome reaction.
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DOI：
10.1091/mbc.11.5.1571
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发表时间：
2000-05
影响因子：
3.3
通讯作者：
C. O'Toole;Christophe Arnoult;A. Darszon;R. Steinhardt;H. Florman
中科院分区：
文献类型：
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作者：
C. O'Toole;Christophe Arnoult;A. Darszon;R. Steinhardt;H. Florman
受精发生在精子顶体反应完成后，这是一种在配子粘附期间触发的分泌事件。ZP3是一种卵透明膜糖蛋白，可使小鼠精子内Ca（2+）浓度持续升高，导致顶体反应。在这里，我们表明，持续的Ca（2+）浓度增加是由于持续激活的Ca（2+）内流机制在ZP3信号转导的后期阶段。这些细胞还具有Ca（2+）储存耗尽激活的Ca（2+）进入途径，该途径在用毒胡萝卜素处理后开放。Thapsigargin和ZP3激活相同的Ca（2+）渗透机制，如荧光猝灭实验和通道拮抗剂所示。这些研究表明，ZP3产生一个持续的Ca（2+）内流通过存储耗尽操作的途径，这驱动胞吐顶体反应。
Fertilization occurs after the completion of the sperm acrosome reaction, a secretory event that is triggered during gamete adhesion. ZP3, an egg zona pellucida glycoprotein, produces a sustained increase of the internal Ca(2+) concentration in mouse sperm, leading to acrosome reactions. Here we show that the sustained Ca(2+) concentration increase is due to the persistent activation of a Ca(2+) influx mechanism during the late stages of ZP3 signal transduction. These cells also possess a Ca(2+) store depletion-activated Ca(2+) entry pathway that is open after treatment with thapsigargin. Thapsigargin and ZP3 activate the same Ca(2+) permeation mechanism, as demonstrated by fluorescence quenching experiments and by channel antagonists. These studies show that ZP3 generates a sustained Ca(2+) influx through a store depletion-operated pathway and that this drives the exocytotic acrosome reaction.