First Identification of β-Lactamases in Antibiotic-Resistant Escherichia coli, Citrobacter freundii, and Aeromonas spp. Isolated in Stream Macroinvertebrates in a Central Italian Region
First Identification of β-Lactamases in Antibiotic-Resistant Escherichia coli, Citrobacter freundii, and Aeromonas spp. Isolated in Stream Macroinvertebrates in a Central Italian Region
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DOI：
10.1089/mdr.2019.0258
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发表时间：
2020-02-26
影响因子：
2.6
通讯作者：
Amicosante, Gianfranco
中科院分区：
文献类型：
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作者：
Segatore, Bernardetta;Piccirilli, Alessandra;Amicosante, Gianfranco
抗生素耐药菌(ARB)广泛存在于自然界，是一个严重的公共和环境问题。在本研究中，我们首次报道了两种具有不同摄食特征的大型无脊椎动物中存在细菌β-内酰胺酶。从水样中分离的弗氏柠檬酸杆菌和大肠杆菌中分别检出A类β-内酰胺酶SHV-1和TEM-1。从捕食性天敌Dina lineata分离的韦氏气单胞菌和嗜水气单胞菌中均发现金属β-内酰胺酶CPHA。只有在从水中分离的大肠杆菌菌株中才能确定存在大的质粒。在所研究的所有菌株中，发现了一种典型的I类整合素整合酶I。在受污染的淡水生境中，ARB和抗生素抗性基因可以通过具有重要生态影响的营养联系传播。通过食物链传播可能有助于抗生素耐药性的传播和转移，不仅在淡水生态系统中，而且在水生栖息地以外。
Antibiotic-resistant bacteria (ARB) are widespread in nature and represent a serious public and environmental problem. In the present study, we report for the first time the presence of bacterial beta-lactamases in two macroinvertebrate species with different feeding traits. The class A beta-lactamases, SHV-1 and TEM-1, were found in Citrobacter freundii isolated from Gammarus elvirae and Escherichia coli from water samples, respectively. The metallo-beta-lactamase CphA was found in Aeromonas veronii and Aeromonas hydrophila strains isolated from the predator Dina lineata. The presence of a large plasmid was ascertained only in E. coli strains isolated from water. In all strains studied, an integrase I typical of class I integrin was found. In contaminated freshwater habitats, ARB and antibiotic resistance genes could be disseminated through trophic links with important ecological implications. Transmission through the food chain may contribute to spreading and transferring antibiotic resistance not only in freshwater ecosystems but also outside the aquatic habitat.