High-Resolution Analysis of Parent-of-Origin Allelic Expression in the Mouse Brain
High-Resolution Analysis of Parent-of-Origin Allelic Expression in the Mouse Brain
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DOI：
10.1126/science.1190830
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发表时间：
2010-08-06
期刊：
影响因子：
56.9
通讯作者：
Dulac, Catherine
中科院分区：
文献类型：
--
作者：
Gregg, Christopher;Zhang, Jiangwen;Dulac, Catherine
基因组印记导致某些基因的父系或母系等位基因的优先表达。我们在小鼠胚胎和成年大脑中进行了全基因组的印记表征。该方法揭示了超过1300个基因座的亲本来源等位基因效应。我们确定了个别基因和特定转录异构体表达的父母偏好，大脑区域之间存在差异。许多印记基因在与进食和动机行为相关的神经系统中表达，并且父母偏好优先靶向控制代谢和细胞粘附的遗传途径。我们观察到一个优惠的母亲的基因表达的贡献，在发育中的大脑和一个主要的父亲的贡献，在成年人的大脑。因此，亲本表达偏好成为大脑中表观遗传调节的主要模式。
Genomic imprinting results in preferential expression of the paternal or maternal allele of certain genes. We have performed a genome-wide characterization of imprinting in the mouse embryonic and adult brain. This approach uncovered parent-of-origin allelic effects of more than 1300 loci. We identified parental bias in the expression of individual genes and of specific transcript isoforms, with differences between brain regions. Many imprinted genes are expressed in neural systems associated with feeding and motivated behaviors, and parental biases preferentially target genetic pathways governing metabolism and cell adhesion. We observed a preferential maternal contribution to gene expression in the developing brain and a major paternal contribution in the adult brain. Thus, parental expression bias emerges as a major mode of epigenetic regulation in the brain.