Steroid Tumor Environment in Male and Female Mice Model of Canine and Human Inflammatory Breast Cancer.
Steroid Tumor Environment in Male and Female Mice Model of Canine and Human Inflammatory Breast Cancer.
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DOI：
10.1155/2016/8909878
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发表时间：
2016
影响因子：
--
通讯作者：
Illera JC
中科院分区：
文献类型：
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作者：
Caceres S;Peña L;Silvan G;Illera MJ;Woodward WA;Reuben JM;Illera JC
犬炎性乳腺癌(IMC)具有与人类炎症性乳腺癌(IBC)相同的临床和组织病理学特征，被认为是研究人类疾病的良好模型。本研究的目的是评估雌性和雄性小鼠复制IMC和IBC肿瘤的能力，并确定荷尔蒙肿瘤环境。为完成这项研究，将60只6-8周龄的雄性和雌性小鼠接种于106IPC-366和SUM149细胞悬液皮下。收集肿瘤和血清，用于激素分析。结果显示，两周后接种IPC-366的男性中90%的人复制了肿瘤，而同时复制肿瘤的女性的比例为100%。在4周后，SUM149在40%的男性而不是80%的女性中复制了肿瘤。这两种细胞系在肺部产生远处转移，比女性的转移率高。EIA分析显示，男性肿瘤的T和SO4E1浓度高于女性肿瘤。血清类固醇水平低于肿瘤中的水平。总之，IBC和IMC雄性小鼠模型是IBC研究的有用工具，而那些循环中的雌激素和肿瘤内激素水平在肿瘤的发生和发展中起着关键作用。
Canine inflammatory mammary cancer (IMC) shares clinical and histopathological characteristics with human inflammatory breast cancer (IBC) and has been proposed as a good model for studying the human disease. The aim of this study was to evaluate the capacity of female and male mice to reproduce IMC and IBC tumors and identify the hormonal tumor environment. To perform the study sixty 6–8-week-old male and female mice were inoculated subcutaneously with a suspension of 106IPC-366 and SUM149 cells. Tumors and serum were collected and used for hormonal analysis. Results revealed that IPC-366 reproduced tumors in 90% of males inoculated after 2 weeks compared with 100% of females that reproduced tumor at the same time. SUM149 reproduced tumors in 40% of males instead of 80% of females that reproduced tumors after 4 weeks. Both cell lines produce distant metastasis in lungs being higher than the metastatic rates in females. EIA analysis revealed that male tumors had higher T and SO4E1 concentrations compared to female tumors. Serum steroid levels were lower than those found in tumors. In conclusion, IBC and IMC male mouse model is useful as a tool for IBC research and those circulating estrogens and intratumoral hormonal levels are crucial in the development and progression of tumors.