Effect of adrenalectomy and dexamethasone on neuropeptide content of dorsal root ganglia in the rat
Effect of adrenalectomy and dexamethasone on neuropeptide content of dorsal root ganglia in the rat
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DOI：
10.1016/0006-8993(91)91347-4
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发表时间：
1991-11
期刊：
影响因子：
2.9
通讯作者：
G. Smith;J. Seckl;W. Sheward;J. Bennie;S. Carroll;H. Dick;A. Harmar
中科院分区：
文献类型：
--
作者：
G. Smith;J. Seckl;W. Sheward;J. Bennie;S. Carroll;H. Dick;A. Harmar
背根神经节（DRG）内的P物质（SP）、降钙素基因相关肽（CGRP）和生长抑素（SS）等神经肽在神经源性炎症和疼痛传递中起重要作用。肾上腺皮质类固醇调节CNS某些区域的神经肽合成，并可能调节神经源性炎症和感觉知觉。我们研究了肾上腺切除术和地塞米松（0.2毫克/公斤/天）治疗对大鼠颈DRG神经肽含量的影响，使用特异性和敏感性放射免疫分析。在对照组动物中，神经肽的差异分布被发现; SP和CGRP含量从C4到C7增加，而SS含量从C4到C7减少。肾上腺切除后10天，颈DRG的平均SS含量显著降低至假手术对照组的79.6 ± 4.5%。而肾上腺切除后10天，SP和CGRP含量显著增加，分别为假手术对照组的134.6 ± 6.9%和132.0 ± 11.6%。肾上腺切除对CGRP和SS的影响可被地塞米松逆转。这些结果提示糖皮质激素影响成年大鼠背根节神经肽含量。
Neuropeptides, including substance P (SP), calcitonin gene-related peptide (CGRP) and somatostatin (SS) in dorsal root ganglia (DRG) may play a role in neurogenic inflammation and pain transmission. Adrenal corticosteroids regulate neuropeptide synthesis in some areas of the CNS and may modulate neurogenic inflammation and sensory perception. We have investigated the effects of adrenalectomy and dexamethasone (0.2 mg/kg/day) treatment on neuropeptide content of rat cervical DRG using specific and sensitive radioimmunoassays. In control animals, a differential distribution of neuropeptide was found; SP and CGRP content increased from C4to C7in contrast to SS content, which decreased from C4to C7. Ten days following adrenalectomy, the mean SS content of cervical DRG decreased significantly to 79.6 ± 4.5% of sham-operated controls. In contrast, SP and CGRP content increased significantly 10 dyas after adrenalectomy to 134.6 ± 6.9% and 132.0 ± 11.6% of sham-operated controls, respectively. The effects of adrenalectomy on CGRP and SS were reversed by administration of dexamethasone. These results suggest that glucocortocoids affect the neuropeptide content of DRG in the adult rat.