Voluntary ethanol drinking in mice and ethanol concentrations in the nucleus accumbens
Voluntary ethanol drinking in mice and ethanol concentrations in the nucleus accumbens
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DOI：
10.1016/j.brainres.2006.12.071
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发表时间：
2007-03-23
期刊：
影响因子：
2.9
通讯作者：
Becker, Howard C.
中科院分区：
文献类型：
--
作者：
Griffin, William C., III;Middaugh, Lawrence D.;Becker, Howard C.
本研究测定了自愿饮酒的C57BL/6J(136)小鼠伏隔核(NAcc)的乙醇浓度。使用Licketer电路来监测消费行为的时间模式，并使用活体微透析技术从NAcc收集系列样本。用气相色谱-火焰离子化检测法测定透析液中的乙醇。在透析期间，小鼠优先摄入足够量的加糖乙醇(类似于3g/kg乙醇)，以在NAcc的透析液中产生低毫摩尔水平的乙醇；水的摄入量可以忽略不计。总体而言，在饮酒2小时期间消耗的酒精总量与NAcc中累积的(以及峰值的)酒精水平呈正相关。此外，舔反应的总数与乙醇的总消耗量呈正相关。此外，NAcc酒精水平的变化与饮酒模式有时间上的联系，在酒精管上出现高舔反应的时期先于大脑酒精水平的峰值。结果表明，B6小鼠在有限的获取时间框架内自愿摄入乙醇会增加NAcc透析液中的乙醇浓度，其方式与乙醇的消耗模式一致。(C)2007 Elsevier B.V.保留所有权利。
The present study determined ethanol concentrations in the nucleus accumbens (NAcc) of C57BL/6J (136) mice voluntarily drinking ethanol using an established limited access paradigm. Lickometer circuits were employed to monitor the temporal pattern of consummatory behavior, and serial samples were collected from the NAcc using in vivo microdialysis techniques. Ethanol in the dialysate was measured by gas chromatography with flame ionization detection. During dialysis, mice preferentially consumed sufficient amounts of sweetened ethanol (similar to 3 g/kg ethanol) to produce low millimolar levels of ethanol in dialysates from the NAcc; water intake was negligible. overall, there was a positive relationship between total amount of ethanol consumed during the 2 h drinking session and cumulative (as well as peak) ethanol levels in NAcc. Additionally, and the total number of licking responses was positively correlated with the total amount of ethanol consumed. Moreover, the change in NAcc ethanol levels was temporally linked to the pattern of ethanol drinking, with periods of high licking responses on the ethanol tube preceding peak brain ethanol levels. The results indicate that the voluntary consumption of ethanol by B6 mice in a limited access time frame elevates ethanol concentration in NAcc dialysates in a manner consistent with the pattern of ethanol consumption. (c) 2007 Elsevier B.V. All rights reserved.