The impact of eotaxin- and IL-5-induced adhesion and transmigration on eosinophil activity markers
The impact of eotaxin- and IL-5-induced adhesion and transmigration on eosinophil activity markers
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DOI：
10.1023/a:1006940109869
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发表时间：
2000-02-01
期刊：
影响因子：
5.1
通讯作者：
Halldén, G
中科院分区：
文献类型：
--
作者：
Fernvik, E;Lundahl, J;Halldén, G
嗜酸性粒细胞在过敏性炎症部位积聚，在哮喘/过敏性疾病中发挥重要作用。嗜酸性粒细胞募集到组织中的机制尚不完全清楚。在本研究中，我们评估了在 IL-5 和嗜酸细胞趋化因子存在的情况下，粘附和/或迁移是否会改变细胞内 ECP 上 CD9、CD11b、β(1)α(4)-整联蛋白和 EG2 表位的表达。我们还研究了 CD9 是否参与粘附过程。使用流式细胞术分析转迁移/粘附至纤连蛋白之前和之后CD9、CD11b和β(1)α(4)-整联蛋白的表面表达以及EG2的细胞内表达。为了评估 CD9 在粘附中的最终作用，将嗜酸性粒细胞与 CD9 单克隆抗体一起预孵育。我们观察到粘附和迁移后，嗜酸性粒细胞上 CD9 的表达减少，CD11b 的表达增加。轮回不会改变β(1)α(4)-整合素或EG2的表达，而粘附导致EG2表达降低。 CD9 抗体降低了嗜酸性粒细胞的粘附特性。嗜酸性粒细胞在粘附和迁移后通过减少 CD9 和增加 CD11b 表达而被激活。当嗜酸性粒细胞粘附于纤连蛋白时，细胞内 ECP 上 EG2 表位的表达降低，可能是由于脱颗粒所致。我们的结果还表明 CD9 参与嗜酸性粒细胞与纤连蛋白的粘附。
Eosinophils accumulate at sites of allergic inflammation, and play important roles in asthma/allergic disorders. The mechanism of eosinophil recruitment into tissues is not fully understood. In this study, we evaluated whether adhesion and/or transmigration, in the presence of IL-5 and eotaxin, alter the expression of CD9, CD11b, the beta(1)alpha(4)-integrin, and the EG2-epitope on intracellular ECP. We also investigated whether CD9 is involved in the adhesion process. With flow cytometry the surface expression of CD9, CD11b and the beta(1)alpha(4)-integrin, and the intracellular expression of EG2, were analyzed before, and after transmigration/adhesion to fibronectin. To evaluate the eventual role of CD9 in adhesion, eosinophils were preincubated with monoclonal antibodies to CD9. We observed decreased expression of CD9, and increased expression of CD11b on eosinophils, after adhesion and transmigration. The transmigration did not change the expression of the beta(1)alpha(4)-integrin or EG2, whereas the adhesion resulted in a decreased EG2 expression. Antibodies to CD9 decreased the adhesion property of eosinophils. The eosinophils are activated after both adhesion and transmigration by means of decreased CD9 and increased CD11b expression. The expression of the EG2-epitope on intracellular ECP was decreased when eosinophils adhered to fibronectin, probably due to degranulation. Our results also indicate that CD9 is involved in the adhesion of eosinophils to fibronectin.