Ontogeny and cellular localization of 125I-labeled basic fibroblast growth factor and 125I-labeled epidermal growth factor binding sites in ovaries from bovine fetuses and neonatal calves.
Ontogeny and cellular localization of 125I-labeled basic fibroblast growth factor and 125I-labeled epidermal growth factor binding sites in ovaries from bovine fetuses and neonatal calves.
复制标题
牛胎儿和新生犊牛卵巢中 125I 标记的碱性成纤维细胞生长因子和 125I 标记的表皮生长因子结合位点的个体发育和细胞定位。
DOI：
10.1095/biolreprod47.5.807
复制
发表时间：
1992
影响因子：
3.6
通讯作者：
M. Sirard
中科院分区：
文献类型：
--
作者：
S. Wandji;G. Pelletier;M. Sirard
我们最近报道，虽然初级和次级卵泡的颗粒细胞中存在特异性的125I-FSH受体，但牛胚胎卵巢的促性腺激素反应性很低，主要由腔前卵泡组成，少数早期腔卵泡。众所周知，许多多肽生长因子对卵泡细胞具有显著的促有丝分裂作用。因此，我们对碱性成纤维细胞生长因子和表皮生长因子结合活性的个体发育和细胞定位进行了放射自显影比较，以评估它们可能参与调节胎儿和新生小牛早期卵泡的生长。胎儿在妊娠180天左右开始卵泡生长。腔前卵泡颗粒细胞的125I-bFGF结合值较高(平均+/-扫描电子显微镜，7.8+/-1.1-9.8+/-0.7颗粒/细胞，0.05-0.15 mm直径)。但在早期有腔卵泡(0.16-3.0 mm)下降至一个恒定水平(5.7+/-1.2粒/细胞)。腔前卵泡的125I-EGF结合值较低，但腔I(0.16-0.5 mm)和腔II(0.6-3.0 mm)卵泡的颗粒细胞和膜内膜分别增加了2.5倍和5.0倍。在闭锁卵泡中，125I-bFGF的特异性结合值较高(10.4+/-0.8个/细胞)，而125I-EGF结合水平显著降低或缺失。所测试的放射性配基均未与原始卵泡显著结合。在与类似大小的卵泡结合的任何配体上，没有年龄相关的差异。这些结果提供了新的证据，证明早在牛腔前阶段，bFGF就参与了颗粒细胞功能的调节。
We have recently reported that although specific 125I-FSH receptors are present in granulosa cells from primary and secondary follicles, gonadotropin responsiveness is very low in ovaries from bovine fetuses, which consist mainly of preantral follicles with few early antral follicles. It is well established that a number of polypeptide growth factors show pronounced mitogenic effects on follicular cells. Therefore, we have compared autoradiographically the ontogeny and cellular localization of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) binding activities to assess their possible involvement in the regulation of early follicular growth in fetuses and neonatal calves. Follicular growth was initiated around Day 180 of gestation in fetuses. 125I-bFGF binding values were high in granulosa cells from preantral follicles (mean +/- SEM, 7.8 +/- 1.1-9.8 +/- 0.7 grains/cell, 0.05-0.15-mm diam.) but decreased in early antral follicles (0.16-3.0 mm) to a constant level (5.7 +/- 1.2 grains/cell). Specific 125I-EGF binding values were low in preantral follicles but showed a 2.5- and 5.0-fold increase in both granulosa cells and the theca interna from antral I (0.16-0.5 mm) and antral II follicles (0.6-3.0 mm), respectively. In atretic follicles, 125I-bFGF specific binding values were high (10.4 +/- 0.8 grains/cell), whereas 125I-EGF binding levels were significantly reduced or absent. None of the radioligands tested bound significantly to primordial follicles. There was no age-related difference in any ligand binding to follicles of comparable size. These results provide novel evidence that bFGF, a potent mitogen, is involved in the regulation of granulosa cell function as early as the preantral stage in cattle.(ABSTRACT TRUNCATED AT 250 WORDS)
培养的大鼠颗粒细胞中表皮生长因子对卵巢孕激素产生的调节。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Jones,PB;WelshJr,TH;Hsueh,AJ
通讯作者：
Hsueh,AJ