Antibacterial and Antifungal Activities of Stereum ostrea, an Inedible Wild Mushroom.
Antibacterial and Antifungal Activities of Stereum ostrea, an Inedible Wild Mushroom.
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DOI：
10.4489/myco.2007.35.4.210
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发表时间：
2007-12
期刊：
影响因子：
1.9
通讯作者：
Lee TS
中科院分区：
文献类型：
--
作者：
Imtiaj A;Jayasinghe C;Lee GW;Lee TS
测定了牡蛎固体培养物的液体培养滤液、水提取物和乙醇提取物对5种细菌和3种植物病原真菌的抑菌活性。以5~300 mg/ml的浓度分别对细菌和真菌进行抑菌试验，测定最小抑菌浓度（MIC）。对枯草芽孢杆菌的MIC为10 mg/ml，对胶孢炭疽菌和宫城炭疽菌的MIC为40 mg/ml。液体培养滤液对革兰氏阳性菌比革兰氏阴性菌更有效，金黄色葡萄球菌是最受抑制的细菌（20.3 mm）。水提物和醇提物对革兰氏阳性菌和革兰氏阴性菌均有抑制作用，且水提物的抑菌效果优于醇提物。水提物和乙醇提取物的抑菌圈分别为23.6和21.0 mm（S.金黄色葡萄球菌）和26.3和22.3 mm（铜绿假单胞菌）。对植物病原真菌的最高和最低抑菌率分别为82.8和14.4。miyabeanus和灰葡萄孢Botrytis cinerea。在水提液中，毛蕊花的PIMG最高为85.2，最低为41.7; miyabeanus和C.胶孢菌属。乙醇提取物对C. miyabeanus比C. gloeosporioides和B.灰叶3种样品中，水提液对供试病原真菌的抑制效果最好。本研究结果表明，S.牡蛎含有抑制细菌和真菌生长的潜在化合物。
Antibacterial and antifungal activities of liquid culture filtrate, water and ethanol extract (solid culture) of Stereum ostrea were evaluated against 5 bacteria and 3 plant pathogenic fungi. To determine the minimal inhibitory concentration (MIC), we studied 5~300 mg/ml concentrations against bacteria and fungi separately. The MIC was 10 mg/ml for Bacillus subtilis and 40 mg/ml for Colletotrichum gloeosporioides and Colletotrichum miyabeanus. Liquid culture filtrate was more effective against Gram positive than Gram negative bacteria, and Staphylococcus aureus was the most inhibited (20.3 mm) bacterium. Water and ethanol extracts were effective against both Gram positive and Gram negative bacteria, and water extract was better than ethanol extract. In water and ethanol extract, inhibition zones were 23.6 and 21.0 mm (S. aureus) and 26.3 and 22.3 mm (Pseudomonas aeruginosa), respectively. For plant pathogenic fungi, the highest and lowest percent inhibition of mycelial growth (PIMG) was found 82.8 and 14.4 against C. miyabeanus and Botrytis cinerea in liquid culture filtrate, respectively. In water extract, the PIMG was found to be the highest 85.2 and lowest 41.7 for C. miyabeanus and C. gloeosporioides, respectively. The inhibitory effect of ethanol extract was better against C. miyabeanus than C. gloeosporioides and B. cinerea. Among 3 samples, water extract was the best against tested pathogenic fungi. This study offers that the extracts isolated from S. ostrea contain potential compounds which inhibit the growth of both bacteria and fungi.