ANTIGENIC VARIATION OF PORCINE TRANSMISSIBLE GASTROENTERITIS VIRUS DETECTED BY MONOCLONAL-ANTIBODIES
ANTIGENIC VARIATION OF PORCINE TRANSMISSIBLE GASTROENTERITIS VIRUS DETECTED BY MONOCLONAL-ANTIBODIES
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DOI：
10.1016/0378-1135(87)90003-4
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发表时间：
1987-06-01
影响因子：
3.3
通讯作者：
MATUMOTO, M
中科院分区：
文献类型：
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作者：
HOHDATSU, T;EIGUCHI, Y;MATUMOTO, M
用纯化病毒抗原免疫BALB/c小鼠，取脾细胞与小鼠骨髓瘤细胞（SP2/O）融合，获得21株与猪传染性胃肠炎病毒（TGE）TO-163株酶联免疫吸附试验（ELISA）反应的单克隆抗体。其中IgG 1型14例，IgG 2a型6例，IgM型1例。所有MC抗体均含有κ型轻链。在这些MC抗体中，发现8种具有针对TO-163菌株的中和（NT）活性。比较7株TGE病毒的NT测试使用我们的面板MC抗体证实了它们的密切的抗原关系，但也揭示了不同的抗原差异的发生。这些结果表明，在TO-163株病毒粒子上可能存在至少6个不同的参与NT反应的表位。这一观点得到了竞争性结合试验的证实。
Mouse myeloma cells (SP2/O) were fused with spleen cells from BALB/c mice immunized with detergent-solubilized antigen of purified virus, and 21 monoclonal (MC) antibodies reactive in enzyme-linked immunosorbent assay with the TO-163 strain of porcine transmissible gastroenteritis (TGE) virus were obtained. Of these MC antibodies, 14, 6 and 1 were IgG1, IgG2a and IgM, respectively. All of the MC antibodies contained light chains of the kappa type. Of these MC antibodies, 8 were found to have neutralization (NT) activity against the TO-163 strain. Comparison of 7 strains of TGE virus by NT tests using our panel of MC antibodies confirmed their close antigenic relationships, but also revealed the occurrence of distinct antigenic differences. These results suggest that there may be at least 6 different epitopes involved in NT reaction on the virion of the TO-163 strain. This notion was confirmed by the competitive binding assay.