Velocity, Processivity, and Individual Steps of Single Myosin V Molecules in Live Cells
Velocity, Processivity, and Individual Steps of Single Myosin V Molecules in Live Cells
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DOI：
10.1016/j.bpj.2009.02.045
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发表时间：
2009-05-20
影响因子：
3.4
通讯作者：
Cappello, Giovanni
中科院分区：
文献类型：
--
作者：
Pierobon, Paolo;Achouri, Sarra;Cappello, Giovanni
我们报告了在自然环境（细胞）中单个肌球蛋白 V 分子的追踪。用量子点标记的肌球蛋白 V 分子被引入活 HeLa 细胞的细胞质中，并在单分子水平上以高空间和时间分辨率记录它们的运动。我们对这种分子转运蛋白的关键参数进行细胞内测量：速度、持续性、步长和停留时间。我们的实验弥补了体外单分子测定与通过跟踪活细胞细胞器推导出的运动特征的间接测量之间的差距。
We report the tracking of single myosin V molecules in their natural environment, the cell. Myosin V molecules, labeled with quantum dots, are introduced into the cytoplasm of living HeLa cells and their motion is recorded at the single molecule level with high spatial and temporal resolution. We perform an intracellular measurement of key parameters of this molecular transporter: velocity, processivity, step size, and dwell time. Our experiments bridge the gap between in vitro single molecule assays and the indirect measurements of the motor features deduced from the tracking of organelles in live cells.