LOCALIZED H-1-NMR MEASUREMENT OF GLUCOSE CONSUMPTION IN THE HUMAN BRAIN DURING VISUAL-STIMULATION
LOCALIZED H-1-NMR MEASUREMENT OF GLUCOSE CONSUMPTION IN THE HUMAN BRAIN DURING VISUAL-STIMULATION
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DOI：
10.1073/pnas.90.21.9896
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发表时间：
1993-11-01
影响因子：
11.1
通讯作者：
SHULMAN, RG
中科院分区：
文献类型：
--
作者：
CHEN, W;NOVOTNY, EJ;SHULMAN, RG
空间定位的H-1 NMR光谱已被应用于测量脑葡萄糖浓度的变化，在8-Hz的光刺激。核磁共振光谱测量是在一个12立方厘米的体积为中心的距状裂，并涵盖初级视觉皮层。葡萄糖水平的平均最大变化为0.34 μ mol.g-1（n = 5）在15分钟，葡萄糖水平已转向静息水平在25分钟。葡萄糖的变化被用来计算增加葡萄糖大脑代谢率在视觉皮层区域的个别受试者通过使用米氏-门滕模型的葡萄糖运输的假设恒定的运输动力学。在光刺激的第一个15分钟内，葡萄糖脑代谢率计算为比未刺激的速率增加22%。在刺激过程中葡萄糖代谢率逐渐降低的模型被提出作为脑葡萄糖和先前测量的乳酸浓度在15分钟后恢复到刺激前值的可能解释。
Spatially localized H-1 NMR spectroscopy has been applied to measure changes in brain glucose concentration during 8-Hz photic stimulation. NMR spectroscopic measurements were made in a 12-cm3 volume centered on the calcarine fissure and encompassing the primary visual cortex. The average maximum change in glucose levels was 0.34 mumol.g-1 (n = 5) at 15 min; glucose level had turned toward resting level at 25 min. The glucose change was used to calculate the increase of glucose cerebral metabolic rate in the visual cortex region for individual subjects by using the Michaelis-Menten model of glucose transport on the assumption of constant transport kinetics. The glucose cerebral metabolic rate was calculated to increase over the nonstimulated rate by 22% during the first 15 min of photic stimulation. A model in which the glucose metabolic rate gradually decreases during stimulation was proposed as a possible explanation for the recovery of brain glucose and previously measured lactate concentrations to prestimulus values after 15 min.