Chondrogenic differentiation of bone marrow‑derived stem cells cultured in the supernatant of elastic cartilage cells.
Chondrogenic differentiation of bone marrow‑derived stem cells cultured in the supernatant of elastic cartilage cells.
复制标题
DOI：
10.3892/mmr.2015.4113
复制
发表时间：
2015-10
影响因子：
3.4
通讯作者：
Xiaodie Zhang;Ke Xue;Jia Zhou;Peng Xu;Huizhen Huang;Kai Liu
中科院分区：
文献类型：
--
作者：
Xiaodie Zhang;Ke Xue;Jia Zhou;Peng Xu;Huizhen Huang;Kai Liu
软骨缺损的修复仍然是外科医生面临的挑战，因为它的自我修复能力很差。软骨组织工程，特别是基于骨髓干细胞的软骨再生，为受损软骨的再生提供了一个有前途的选择。虽然从骨髓干细胞中生产组织工程软骨似乎是一种可行的方法，但构建某些类型的软骨，包括弹性软骨，仍然很困难。因此，本研究探讨了通过在弹性软骨细胞的上清液中培养骨髓源性干细胞（BMSCs）来构建弹性软骨的可行性。从新生猪耳廓软骨中获得弹性软骨细胞，从猪骨髓中分离骨髓间充质干细胞。收集软骨细胞上清培养骨髓间充质干细胞。在不同的时间点，通过大体观察、组织学检查和定量聚合酶链反应来评估BMSCs的分化。随着培养时间的延长，BMSCs由梭形细胞向多角形细胞转化。在弹性软骨细胞上清液中培养的细胞中观察到II型胶原和弹性蛋白的表达，而在对照细胞中未观察到表达。此外，在弹性软骨细胞上清液中培养的细胞中，I型胶原和X型胶原的表达下调。弹性软骨细胞培养上清可促进BMSCs向弹性软骨细胞分化，这可能是一种有前途的构建组织工程软骨的方法。
Repair of cartilage defects remains a challenge for surgeons, owing to its poor self‑repairing capacity. Cartilage tissue engineering, particularly marrow stem cell‑based cartilage regeneration, provides a promising option for the regeneration of damaged cartilage. Although producing tissue‑engineered cartilage from marrow stem cells appeared to be a feasible method, constructing certain sub‑types of cartilage, including elastic cartilage, remains difficult. Therefore, the present study explored the feasibility of constructing elastic cartilage by culturing bone marrow‑derived stem cells (BMSCs) in the supernatant of elastic cartilage cells to generate elastic cartilage. The elastic cartilage cells were obtained from the auricle cartilage of a newborn pig, and BMSCs were isolated from pig bone marrow aspirate. The supernatant of the chondrocytes was collected and then used to the culture BMSCs. At various time‑points, the differentiation of BMSCs was evaluated by gross view, histological examination and quantitative polymerase chain reaction. BMSCs changed from spindle‑shaped cells into polygonal cells with increasing culture time. The expression of collagen II and elastin was observed in the cells cultured in the supernatant of elastic chondrocytes, while no expression was observed in the control cells. Furthermore, the expression of collagen I and collagen X was downregulated in the cells cultured in the supernatant of elastic cartilage cells. The supernatant of elastic cartilage cells promoted the differentiation of BMSCs into elastic cartilage cells, which may be a promising method for constructing certain sub‑types of tissue‑engineered cartilage.