Protein adsorption on poly(N-vinylpyrrolidone)-modified silicon surfaces prepared by surface-initiated atom transfer radical polymerization.
Protein adsorption on poly(N-vinylpyrrolidone)-modified silicon surfaces prepared by surface-initiated atom transfer radical polymerization.
复制标题
DOI：
10.1021/la8037523
复制
发表时间：
2009-02
期刊：
影响因子：
--
通讯作者：
Zhaoqiang Wu;Hong Chen;Xiaoli Liu;Yanxia Zhang;Dan Li;He Huang
中科院分区：
文献类型：
--
作者：
Zhaoqiang Wu;Hong Chen;Xiaoli Liu;Yanxia Zhang;Dan Li;He Huang
以CuCl/5，7，7，12，1,4，1,4-六甲基-1，4，8，11-四氮杂环十四烷（Me 6 TATD）为催化剂，1，4-二氧六环/水为溶剂，通过表面引发原子转移自由基聚合（SI-ATRP），在硅表面制备了可控的聚乙烯吡咯烷酮（PVP）接枝硅表面.表面上的PVP层的厚度随反应时间的增加，表明从硅表面的N-乙烯基吡咯烷酮（NVP）的ATRP接枝是一个良好的控制过程。采用水接触角和X射线光电子能谱（XPS）对改性后的表面进行了表征。使用放射性标记方法评价PVP接枝表面的蛋白质吸附性质。与未改性的硅表面相比，PVP厚度为15.06 nm的Si-PVP 60表面降低了纤维蛋白原，人血清白蛋白（HSA）和溶菌酶的吸附水平分别为75%，93%和81%。此外，纤维蛋白原的吸附水平随着PVP厚度的增加而逐渐降低。然而，当PVP层厚于临界厚度13.45 nm时，纤维蛋白原吸附没有显著差异。
Well-controlled poly(N-vinylpyrrolidone) (PVP)-grafted silicon surfaces were prepared by surface-initiated atom transfer radical polymerization (SI-ATRP) with 1,4-dioxane/water mixtures as solvents and CuCl/5,7,7,12,14,14-hexamethyl-1,4,8,11-tetraazacyclotetradecane (Me6TATD) as a catalyst. The thickness of the PVP layer on the surface increased with reaction time, suggesting that the ATRP grafting of N-vinylpyrrolidone (NVP) from the silicon surfaces was a well-controlled process. The water contact angle and X-ray photoelectron spectroscopy (XPS) were used to characterize the modified surfaces. The protein adsorption property of the PVP-grafted surfaces was evaluated using a radiolabeling method. Compared with unmodified silicon surfaces, a Si-PVP60 surface with a PVP thickness of 15.06 nm reduced the level of adsorption of fibrinogen, human serum albumin (HSA), and lysozyme by 75, 93, and 81%, respectively. Moreover, the level of fibrinogen adsorption decreases gradually with an increase in PVP thickness. However, no significant difference in fibrinogen adsorption was found when the PVP layer was thicker than the critical thickness of 13.45 nm.