Engineering micropatterned surfaces for the coculture of hepatocytes and Kupffer cells.
Engineering micropatterned surfaces for the coculture of hepatocytes and Kupffer cells.
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用于肝细胞和枯否细胞共培养的微图案表面工程。
DOI：
10.1002/jbm.a.30399
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Coger,RobinN
中科院分区：
文献类型：
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作者：
Zinchenko,YekaterinaS;Coger,RobinN
生物人工肝（BAL）设备用于从药物测试到临时肝脏替代的应用。这些设备的能力可以通过优化BAL能够执行的肝细胞功能范围来提高。实现这一点的一种方法是设计BAL，使其在肝细胞和非实质细胞之间建立通信。为了理解这些异型相互作用如何在BAL设计中有利地利用，首先需要建立允许多种细胞类型的受控相互作用的培养环境。这是当前研究的目标，其重点是肝细胞与枯否细胞的微模式共培养。微图案化技术依赖于聚二甲基硅氧烷（PDMS）膜，以在接种细胞群之前实现ECM的各种二维构型。制造PDMS膜的简单且廉价的方法不同于文献中报道的方法，并且在当前研究中详细描述。为了证明该方法的成功，所得到的微图案的表面表征，以及形态和功能的结果也被提出。© 2005 Wiley Periodicals，Inc.生物医学材料研究杂志，2005年
Bioartificial liver (BAL) devices are used for applications ranging from pharmaceutical testing to temporary liver replacement. The capabilities of these devices can be improved by optimizing the range of hepatocyte functions that the BAL is able to perform. One means of achieving this is to design the BAL such that it establishes communication between hepatocytes and nonparenchymal cells. To understand how these heterotypic interactions can be favorably utilized in BAL design, it is first necessary to establish a culture environment that permits the controlled interactions of multiple cell types. This is the goal of the current study, which focuses on micropatterned cocultures of hepatocytes with Kupffer cells. The micropatterning technique relies on a polydimethylsiloxane (PDMS) membrane to achieve various two‐dimensional configurations of the ECM prior to seeding the cell populations. The easy and inexpensive method of making the PDMS membranes differs from that reported in the literature and is detailed in the current study. To demonstrate the success of the method, surface characterization of the resultant micropatterns, as well as morphological and functional results are also presented. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005