Cytoskeletal changes induced by two avian sarcoma viruses: UR2 and Rous sarcoma virus.
Cytoskeletal changes induced by two avian sarcoma viruses: UR2 and Rous sarcoma virus.
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两种禽肉瘤病毒引起的细胞骨架变化：UR2 和劳斯肉瘤病毒。
DOI：
10.1016/0042-6822(84)90247-2
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发表时间：
1984
期刊：
影响因子：
3.7
通讯作者：
Balduzzi,PC
中科院分区：
文献类型：
--
作者：
Notter,MF;Balduzzi,PC
通过免疫荧光检查UR2转化的细胞，并与对照细胞和劳斯肉瘤病毒(RSV)转化的细胞进行比较。通常在 RSV 转化细胞中解聚的肌动蛋白和微管蛋白似乎不受 UR2 转化的影响。细胞表面纤连蛋白通常从 RSV 感染的细胞中丢失，但在 UR2 转化的细胞上比在正常细胞上出现更丰富。纽蛋白存在于 UR2 转化细胞和对照成纤维细胞的粘附斑中，但扩散在 RSV 转化细胞的细胞质中。 [35S]蛋氨酸标记的纤连蛋白和从正常细胞和转化细胞的裂解物中免疫沉淀的纽蛋白的聚丙烯酰胺凝胶电泳表明，在标记期间合成的细胞相关纤连蛋白在RSV转化细胞中减少了60%，但在UR2转化细胞中以正常量出现。然而，对正常细胞和转化细胞的上清液中释放的放射性标记纤连蛋白的免疫沉淀显示，与未感染的培养物相比，UR2转化细胞的液体中纤连蛋白的量减少，但RSV感染的细胞的培养基中纤连蛋白的量显着增加。这些数据表明，与正常细胞相比，更多的纤连蛋白与 UR2 转化细胞的表面结合，但很容易从 RSV 转化细胞中释放。在 RSV 和 U112 转化中，纽蛋白均比正常水平减少约 50%。细胞。使用病毒体结构蛋白 (gag) 抗体进行的免疫荧光研究表明，UR2 转化细胞的细胞核不发出荧光。这表明 UR2 的转化蛋白 p68ros 存在细胞质位置或膜关联，其中含有 gag 决定簇。总体而言，这些数据表明，成纤维细胞主要细胞骨架蛋白的变化对于细胞的肿瘤特性并不重要，而是转化的表型表达，因为在体内引起肿瘤的UR2仅诱导体外转化细胞的微小细胞骨架改变。
UR2-transformed cells were examined by immunofluorescence and compared to control cells and cells transformed by Rous Sarcoma Virus (RSV). Actin and tubulin which are normally depolymerized in RSV-transformed cells appeared to be unaffected by UR2 transformation. Cell surface fibronectin which is normally lost from RSV-infected cells, appears more abundantly on UR2-transformed cells than on normal cells. Vinculin was shown to be in adhesion plaques in UR2-transformed cells as well as in control fibroblasts but diffuse in the cytoplasm of RSV-transformed cells. Polyacrylamide gel electrophoresis of [35S]methionine-labeled fibronectin and vinculin immunoprecipitated from lysates of normal and transformed cells indicated that cell associated fibronectin synthesized during the labeling period is reduced by 60% in RSV-transformed cells but occurs in normal amounts in UR2-transformed cells. However, immunoprecipitation of radiolabeled fibronectin released in supernatant fluids of normal and transformed cells showed a decreased amount of fibronectin in fluids from UR2-transformed cells, but a considerable increase in the medium from RSV-infected cells as compared to uninfected cultures. These data suggest that more fibronectin binds to the surface of UR2-transformed cells then to normal cells, but is readily released from RSV-transformed cells. Vinculin was reduced by about 50% of normal levels in both RSV- and U112-transformed. cells. Immunofluorescence studies using antibody to virion structural proteins (gag) show that the nuclei of UR2-transformed cells are not fluorescent. This indicates a cytoplasmic location or membrane association for p68rosthe transforming protein of UR2, which containsgagdeterminants. Overall, these data suggest that changes in the major cytoskeletal proteins of fibroblasts are not essential for the neoplastic properties of cells but are rather a phenotypic expression of transformation, since UR2, which causes tumorsin vivo, induces only minor cytoskeletal alterations of cells transformedin vitro.