Photochemical repair of Achilles tendon rupture in a rat model
Photochemical repair of Achilles tendon rupture in a rat model
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DOI：
10.1016/j.jss.2004.09.019
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发表时间：
2005-04-01
影响因子：
2.2
通讯作者：
Redmond, RW
中科院分区：
文献类型：
--
作者：
Chan, BP;Amann, C;Redmond, RW
背景光化学组织粘合（PTB）是一种用于粘合或密封组织表面的新兴技术，其需要光和光敏染料来实现其效果。在大鼠模型中评估PTB用于肌腱修复的潜力。离体测定牛肌腱的光学性质，以测量作为波长的函数的光穿透深度，并建立用于PTB修复断裂肌腱的剂量学参数。然后在Sprague-Dawley大鼠体内测试PTB修复横断肌腱。治疗后14天使用应变仪测量修复强度。在514 nm处，肌腱中的有效穿透深度估计为0.68 mm。PTB治疗机械性断裂肌腱后，显著的粘合取决于光和染料的存在，并在125 J/cm（2）的能量密度下达到平台强度。在随后的研究PTB修复横断大鼠跟腱的体内研究中，在照射后立即和修复后7天和14天时测量了断裂修复肌腱所需的极限应力。结果显示，对照组和PTB治疗组之间的极限应力差异在治疗后即刻和修复后7天（p = 0.04）但不包括14天（p = 0.75）时具有统计学显著性。PTB在修复的早期阶段为肌腱修复提供了益处，值得进一步研究，作为骨科手术中肌腱修复的潜在手术辅助手段。(C)2005年爱思唯尔公司All rights reserved.
Background. Photochemical tissue bonding (PTB) is an emerging technique for bonding or sealing tissue surfaces that requires light and a photoactive dye for its effect. The potential of PTB for tendon repair was assessed in a rat model.Materials and methods. The optical properties of bovine tendon were determined ex vivo to gauge the depth of light penetration as a function of wavelength and dosimetry parameters were established for PTB repair of ruptured tendon. PTB was then tested in vivo to repair transected tendons in Sprague-Dawley rats. Repair strengths were measured using a strain gauge up to 14 days post treatment.Results. The effective penetration depth in tendon was estimated to be 0.68 mm at 514 nm. Following PTB treatment of mechanically ruptured tendon, significant bonding was dependent on the presence of both light and dye and attained a plateau strength at a fluence of 125 j/cm(2). In a subsequent in vivo study to investigate PTB for repair of transected rat Achilles tendon, the ultimate stress required to break the repaired tendon was measured immediately after irradiation and at 7 and 14 days post-repair. Results showed that the difference in the ultimate stress between control and PTB treatment groups was statistically significant immediately after treatment and at 7 days (p = 0.04) but not 14 days (p = 0.75) post-repair.Conclusions. PTB provides a benefit to tendon repair at early stages in repair and is worthy of further investigation as a potential surgical adjunct for tendon repair in orthopedic surgeries. (C) 2005 Elsevier Inc. All rights reserved.