Label-free visualization of fruit lignification: Raman molecular imaging of loquat lignified cells.
Label-free visualization of fruit lignification: Raman molecular imaging of loquat lignified cells.
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果实木质化的无标记可视化：枇杷木质化细胞的拉曼分子成像
DOI：
10.1186/s13007-018-0328-1
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发表时间：
2018
期刊：
影响因子：
5.1
通讯作者：
Chen K
中科院分区：
文献类型：
--
作者：
Zhu N;Wu D;Chen K
研究背景果肉木质化可导致果实硬度增加，这在许多水果中都有报道。了解果实木质化的发生机制，对于优化采后贮藏策略，减少果实采后品质劣变具有重要意义。特别是在细胞水平上研究果实中木质素的沉积，为阐明果实木质化的机理提供了新的视角。本研究的主要目的是建立一种利用拉曼显微光谱技术在细胞水平上描述果实木质化的方法。而白沙枇杷果肉中几乎未检测到这种特殊的脂细胞。木质素细胞的主要拉曼谱带主要是木质素（1664、1628、1603、1467和1272 cm−1）、纤维素（1383、1124和1098 cm−1）和果胶（852和1740 cm−1）。谱带强度相关分析表明，在1335 cm-1处的峰在以前的工作中被指定为木质素或纤维素，这与木质素细胞的木质素有关。多峰高斯拟合成功地将1550-1700 cm-1的重叠指纹峰分解为三个独立的峰，分别归属于木质素的不同官能团。木质细胞的空间分辨拉曼图像显示木质素和纤维素遍布整个木质细胞，果胶主要分布在细胞角部，薄壁细胞中木质素含量很少。此外，间苯三酚-盐酸染色和自发荧光分析证实了木质素分布的拉曼显微analys.ConclusionsA程序的主要成分的果肉细胞的同时可视化没有标记的高分辨率拉曼显微光谱的结果。利用拉曼显微成像技术，我们可以增加一个微观水平的细胞组成，为详细的分子理解枇杷木质化。这种方法还可用于化学监测其他水果和蔬菜在成熟过程或采后贮藏期间的质构变化。
BackgroundFlesh lignification, leading to increased fruit firmness, has been reported in several kinds of fruit. Understanding the mechanisms underlying fruit lignification is important to optimize the postharvest storage strategies and reduce the quality deterioration of postharvest fruit. Especially cellular level investigation of lignin deposition in fruits provides novel insight for deciphering the mechanisms underlying fruit lignification. The primary objective of this study was to establish a procedure of using Raman microspectroscopy technique to depict fruit lignification at the cell level.ResultsLignified cells, a special kind of cells contained high lignin content, were found abundantly scattered in red-fleshed ‘Luoyangqing’ loquat. Whereas these special lignified cells were barely detected in ‘Baisha’ loquat flesh. Dominant Raman bands of lignified cells were found primarily attributed to lignin (1664, 1628, 1603, 1467, and 1272 cm−1), cellulose (1383, 1124 and 1098 cm−1) and pectin (852 and 1740 cm−1). The band intensity correlation analysis indicated the peak at 1335 cm−1assigned to either lignin or cellulose in previous works was related to lignin for the lignified cells. Multi-peaks Gaussian fitting successfully resolved the overlapped fingerprint peaks of lignin in 1550–1700 cm−1into three independent peaks, which were assigned to different functional groups of lignin. Furthermore, the spatially resolved Raman images of lignified cells were generated, indicating that lignin and cellulose saturated the whole lignified cells, pectin mainly located in the cell corner, and the parenchyma cells contained little lignin. In addition, both phloroglucinol-HCl staining and autofluorescence analysis confirmed the results of lignin distribution of Raman microscopic analysis.ConclusionsA procedure for the simultaneous visualization of the main components of the flesh cells without labeling by high-resolution Raman microspectroscopy has been established. With Raman microscopic imaging technique, we can add a microscopic level to cell compositions, essential for a detailed molecular understanding of loquat lignification. Such method can be further used to chemically monitor the textural changes during the ripening process or postharvest storage of other fruits and vegetables.
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影响因子：
6.9
作者：
Blanco-Portales, R;Medina-Escobar, N;Muñoz-Blanco, J
通讯作者：
Muñoz-Blanco, J
影响因子：
32.5
作者：
DeMartini, Jaclyn D.;Pattathil, Sivakumar;Wyman, Charles E.
通讯作者：
Wyman, Charles E.
银杏树（Ginkgo biloba L.）正常木材和压缩木材中的纤维素结构和木质素分布
影响因子：
11.4
作者：
Andersson, Seppo;Wang, Yurong;Saranpaa, Pekka
通讯作者：
Saranpaa, Pekka
影响因子：
7
作者：
Ding, CK;Chachin, K;Imahori, Y
通讯作者：
Imahori, Y
影响因子：
11.2
作者：
Femenia, A;Garcia-Conesa, M;Rossello, C
通讯作者：
Rossello, C