Mechanisms of the relaxant action of ketamine on isolated porcine trachealis muscle.
Mechanisms of the relaxant action of ketamine on isolated porcine trachealis muscle.
复制标题
氯胺酮对离体猪气管肌松弛作用的机制。
DOI：
10.1093/bja/71.4.544
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发表时间：
1993
影响因子：
9.8
通讯作者：
Rehder,K
中科院分区：
文献类型：
--
作者：
Wilson,LE;Hatch,DJ;Rehder,K
氯胺酮是一种有效的支气管扩张剂，但其作用方式尚不清楚。本文研究了氯胺酮对离体猪气管肌外周迷走神经运动通路的影响。以1,1-二甲基-4-苯基碘化哌嗪选择性刺激神经节内的突触后烟碱胆碱能受体，以电场刺激神经节后神经纤维（在六甲铵存在下），以乙酰胆碱（在河豚毒素存在下）刺激毒蕈碱胆碱能受体。氯胺酮10−4mol l l−1使乙酰胆碱的浓度-响应曲线（P<0.02）和电场刺激（P<0.001）向右移动，使1,1-二甲基-4-苯基-碘化哌嗪的响应消失（P<0.02）。氯胺酮还引起乙酰胆碱预收缩肌肉条的浓度依赖性松弛。心得安不受影响。氯他宁在没有或存在阿托品的情况下，放松与氯化钾一起预收缩的肌条。我们得出结论，氯胺酮与周围迷走神经的相互作用是通过降低神经节内突触后烟碱受体的兴奋性，以及通过影响毒蕈碱受体、平滑肌，或两者兼而有之。β -2肾上腺素受体不参与松弛机制。J.阿纳斯特，1993；71: 544 - 550)
Ketamine is a potent bronchodilator, but its mode of action is unclear. We have studied the effect of ketamine on the peripheral vagus nerve motor pathway of isolated porcine trachealls muscle. Posts ynaptic nicotinic cholinergic receptors of the intramural ganglia were stimulated selectively with 1,1-dimethyl-4-phenyl-piperazinium iodide, postganglionic nerve fibres with electrical field stimulation (in the presence of hexamethonium) and muscarinic cholinergic receptors with acetylcholine (in the presence of tetrodotoxin). Ketamine 10−4mol litre−1significantly shifted the concentration-response curves of acetylcholine (P< 0.02) and electrical field stimulation (P<0.001) to the right and abolished the response to 1,1-dimethyl-4-phenyl-piperazinium iodide (P<0.02). Ketamine also caused a concentration-dependent relaxation of muscle strips precontracted with acetylcholine. This was unaffected by propranolol. Ketarnine relaxed muscle strips precontracted with potassium chloride, in the absence and presence of atropine. We conclude that ketamine interacts with the peripheral vagus nerve by decreasing the excitability of the posts ynaptic nicotinic receptors of the intramural ganglia, and by affecting the muscarinic receptor, smooth muscle, or both. Beta-2adrenoceptors are not involved in the mechanism of relaxation.(Br. J. Anaesth. 1993; 71: 544–550)