Polymorphism of avian sarcoma virus src proteins
Polymorphism of avian sarcoma virus src proteins
复制标题
禽肉瘤病毒src蛋白多态性
DOI：
10.1128/jvi.30.1.190-200.1979
复制
发表时间：
1979
影响因子：
5.4
通讯作者：
B. Sefton
中科院分区：
文献类型：
--
作者：
K. Beemon;T. Hunter;B. Sefton
比较了7种不同禽肉瘤病毒的src基因产物。在两株施密特-鲁平株、三株布拉格株、布赖恩株和布拉迪斯拉发77株禽肉瘤病毒中，病毒体RNA的体外翻译产生了明确鉴定为p60 src的产物。这七个p60 src蛋白在十二烷基硫酸钠-聚丙烯酰胺凝胶中的电泳迁移率的差异，对应于表观分子量范围从56，000到60，500的变化，进行了观察。抗原变异性也被发现，只有三个测试的七种病毒编码的p60 src，这是沉淀的抗血清来源于兔子引起的施密特Ruppin株劳氏肉瘤病毒的肿瘤。检查含甲硫氨酸的胰蛋白酶肽的7个; 60 src蛋白质的二维映射显示四个常见的肽，但显着的变异性，在每个蛋白质中的5至8个其他肽。在不同病毒株之间和病毒株内，观察到p60 src肽图的明显差异。在检查的三种情况下，发现在转化细胞中合成的p60 src与体外合成的p60 src基本相同。因此，禽肉瘤病毒p60 src蛋白存在明显的多态性。
The src gene products of seven different avian sarcoma viruses were compared. In vitro translation of virion RNA yielded products identified unambiguously as p60src in the case of two stocks of the Schmidt-Ruppin strain, three stocks of the Prague strain, the Bryan strain, and the Bratislava 77 strain of avian sarcoma virus. Differences in the electrophoretic mobility of these seven p60src proteins in sodium dodecyl sulfate-polyacrylamide gels, corresponding to variation in the apparent molecular weights ranging from 56,000 to 60,500, were observed. Antigenic variability was also found; only three of the seven viruses tested encoded a p60src, which was precipitated by antisera derived from rabbits bearing tumors induced by the Schmidt-Ruppin strain of Rous sarcoma virus. Examination of the methionine-containing tryptic peptides of the seven ;60src proteins by two-dimensional mapping revealed four common peptides but marked variability in the five to eight other peptides in each protein. Clear differences in the peptide maps of p60src were observed, both between different strains of virus and within strains. In the three cases examined, p60src synthesized in transformed cells was found to be essentially identical to that synthesized in vitro. We conclude that there is significant polymorphism in the p60src proteins of the avian sarcoma viruses.