RAPID INTERPHASE AND METAPHASE ASSESSMENT OF SPECIFIC CHROMOSOMAL CHANGES IN NEUROECTODERMAL TUMOR-CELLS BY INSITU HYBRIDIZATION WITH CHEMICALLY MODIFIED DNA PROBES
RAPID INTERPHASE AND METAPHASE ASSESSMENT OF SPECIFIC CHROMOSOMAL CHANGES IN NEUROECTODERMAL TUMOR-CELLS BY INSITU HYBRIDIZATION WITH CHEMICALLY MODIFIED DNA PROBES
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DOI：
10.1016/0014-4827(88)90325-4
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发表时间：
1988-06-01
影响因子：
3.7
通讯作者：
MANUELIDIS, L
中科院分区：
文献类型：
--
作者：
CREMER, T;TESIN, D;MANUELIDIS, L
重复的DNA组成的异染色质的人类1号和18号染色体被用作探针，在非放射性原位杂交实验，以确定特定的数字和结构的染色体畸变在三个人胶质瘤细胞系和一个神经母细胞瘤细胞系。这些肿瘤细胞系的间期核和正常二倍体细胞核中检测到的斑点数量与原位杂交特异标记的染色体中期计数相关。通过双杂交实验，可快速、可靠地检测肿瘤细胞系中的非整倍体染色体数目，并可在特定的肿瘤细胞群体中检测出具有奇异表型和染色体构成的罕见细胞。即使在次优或罕见的染色体扩散中，也描述了特定的染色体畸变。随着更广泛的探针集变得可用，这种方法将变得越来越强大，用于揭示肿瘤细胞中的各种遗传改变及其进展。
Repeated DNAs from the constitutive heterochromatin of human chromosomes 1 and 18 were used as probes in nonradioactive in situ hybridization experiments to define specific numerical and structural chromosome aberrations in three human glioma cell lines and one neuroblastoma cell line. The number of spots detected in interphase nuclei of these tumour cell lines and in normal diploid nuclei correlated well with metaphase counts of chromosomes specifically labeled by in situ hybridization. Rapid and reliable assessments of aneuploid chromosome numbers in tumor lines in double hybridization experiments were achieved, and rare cells with bizarre phenotype and chromosome constitution could be evaluated in a given tumor cell populaion. Even with suboptimal or rare chromosome spreads specific chromosome aberrations were delineated. As more extensive probe sets become available this approach will become increasingly powerful for uncovering various genetic alterations and their progression in tumor cells.