Effect of supplemental ornithine on wound healing
Effect of supplemental ornithine on wound healing
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DOI：
10.1006/jsre.2002.6471
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发表时间：
2002-08-01
影响因子：
2.2
通讯作者：
Barbul, A
中科院分区：
文献类型：
--
作者：
Shi, HP;Fishel, RS;Barbul, A
背景。补充精氨酸已被证明可以增强伤口愈合，特别是胶原蛋白的合成。鸟氨酸是精氨酸在尿素循环中的主要代谢产物，具有精氨酸的许多生物药理学作用。本研究探讨了鸟氨酸补充剂对伤口愈合的影响，并试图描述其可能的机制。方法。野生型 (WT) 和 iNOS 敲除 (KO) 小鼠被随机分配接受正常食物和自来水或分别补充 0.5% 鸟氨酸 (w/w) 的食物和水。所有动物均进行背侧皮肤中线切口，并将聚乙烯醇海绵植入皮下袋中。术后第14天处死动物。收获背部伤口用于断裂强度测定，同时测定伤口海绵的羟脯氨酸含量、总伤口液体氨基酸和亚硝酸盐/硝酸盐(NOx)浓度。结果。饮食中补充鸟氨酸可增强 WT 和 KO 小鼠的伤口断裂强度和胶原蛋白沉积。这伴随着伤口液中脯氨酸和鸟氨酸水平的增加，但精氨酸、瓜氨酸或氮氧化物水平没有增加。结论。这项研究的结果表明，补充鸟氨酸可以增强 WT 和 KO 小鼠的伤口愈合。这表明鸟氨酸对伤口愈合的作用与 iNOS 途径无关。 (C) 2002 Elsevier Science (USA).
Background. Supplemental arginine has been shown to enhance wound healing, in particular collagen synthesis. Ornithine is the main metabolite of arginine in the urea cycle and shares many of the biopharmacologic effects of arginine. The present study examines the effect of ornithine supplementation on wound healing and attempts to describe its possible mechanism.Methods. Wild type (WT) and iNOS knockout (KO) mice were randomized to receive either normal chow and tap water or chow and water each supplemented with 0.5% ornithine (w/w). All animals underwent a midline dorsal skin incision with implantation of polyvinyl alcohol sponges into subcutaneous pockets. On postoperative day 14 the animals were sacrificed. The dorsal wound was harvested for breaking strength determination while the wound sponges were assayed for hydroxyproline content, total wound fluid amino acid, and nitrite/nitrate (NOx) concentration.Results. Dietary ornithine supplementation enhanced wound breaking strength and collagen deposition in both WT and KO mice. This was accompanied by increased wound fluid proline and ornithine levels but not arginine, citrulline, or NOx levels.Conclusions. The results from this study demonstrate that ornithine supplementation enhances wound healing in both WT and KO mice. This suggests that ornithine's effect on wound healing is independent of the iNOS pathway. (C) 2002 Elsevier Science (USA).