Skeletal myogenic progenitors in the endothelium of lung and yolk sac
Skeletal myogenic progenitors in the endothelium of lung and yolk sac
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DOI：
10.1016/s0014-4827(03)00314-8
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发表时间：
2003-11-01
影响因子：
3.7
通讯作者：
Cossu, G
中科院分区：
文献类型：
--
作者：
De Angelis, MGC;Balconi, G;Cossu, G
我们以前的研究表明，可克隆的骨骼肌细胞可以从胚胎主动脉中获得，但在更成熟和结构的胎儿主动脉中变得非常罕见。本研究的目的是调查在胎儿和出生后的发育过程中，这些肌源性祖细胞是否逐渐消失，或者可能与微血管区有关，从而分布到几乎所有组织。为了验证这一假设，我们使用来自表达横纹肌特异性报告基因（LacZ）的转基因株系的FI胚胎（或小鼠）与表达不同内皮特异性报告基因（GFP）的转基因株系杂交。内皮细胞从卵黄囊（E11）和肺（E11, E17, P1， P10和P60）中分离出来，这两个器官在胚胎学上与旁轴中胚层无关，血管丰富，缺乏骨骼肌。内皮细胞通过磁珠选择（CD31/PECAM-1(+)）或细胞分选（Tie2-GFP(+)）纯化，然后在体外和体内挑战其骨骼肌生成潜力。结果表明，卵黄囊和肺都含有祖细胞，它们表达内皮标记，并具有骨骼肌生成的潜力，当它们在体外分化成肌细胞和体内再生肌肉时，它们就会显示出来。这些血管相关细胞的数量（或产生骨骼肌的能力）随着年龄的增长而迅速减少，在成熟动物中非常低，可能与成年哺乳动物组织的再生能力降低有关。(C) 2003 Elsevier Inc.版权所有。
We previously showed that clonable skeletal myogenic cells can be derived from the embryonic aorta but become very rare in the more mature and structured fetal aorta. The aim of this study was to investigate whether, during fetal and postnatal development, these myogenic progenitors progressively disappear or may rather associate with the microvascular district, being thus distributed to virtually all tissues. To test this hypothesis, we used FI embryos (or mice) from a transgenic line expressing a striated muscle-specific reporter gene (LacZ) crossed with a transgenic line expressing a different endothelial-specific reporter genes (GFP). Endothelial cells were isolated from yolk sac (at E 11) and lung (at E11, E17, P1, P10, and P60), two organs embryologically unrelated to paraxial mesoderm, rich in vessels, and devoid of skeletal muscle. Endothelial cells, purified by magnetic bead selection (CD31/PECAM-1(+)) or cell sorting (Tie2-GFP(+)) were then challenged for their skeletal myogenic potential in vitro and in vivo.The results demonstrated that both yolk sac and lung contain progenitor cells, which express endothelial markers and are endowed with a skeletal myogenic potential that they reveal when in the presence of differentiating myoblasts, in vitro, and regenerating muscle, in vivo.The number (or potency to generate skeletal muscle) of these vessels associated cells decreases rapidly with age and is very low in mature animals, possibly correlating with reduced regenerative capacity of adult mammalian tissues. (C) 2003 Elsevier Inc. All rights reserved.