In vitro immunomodulation by pentachlorophenol in phagocytes from an estuarine teleost, Fundulus heteroclitus, as measured by chemiluminescence activity.
In vitro immunomodulation by pentachlorophenol in phagocytes from an estuarine teleost, Fundulus heteroclitus, as measured by chemiluminescence activity.
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通过化学发光活性测量，五氯苯酚对河口硬骨鱼（Fundulusheteroclitus）吞噬细胞的体外免疫调节作用。
DOI：
10.1007/bf00214338
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发表时间：
1993
影响因子：
4
通讯作者：
Anderson,RS
中科院分区：
文献类型：
--
作者：
Roszell,LE;Anderson,RS
采用化学发光法研究了五氯苯酚（PCP）对河口硬骨鱼底栖动物白细胞产生活性氧中间产物（ROI）的影响。在吞噬诱导的呼吸爆发期间测量两个参数：峰值CL（最大值）和总CL（诱导值的时间总和）。在亚致死剂量的分析级（A-）或技术级（T-）五氯苯酚存在的情况下，培养从原肾中获得的吞噬细胞。据报告，在哺乳动物体内，工业五氯苯酚比甲型五氯苯酚具有更强的免疫抑制作用;这一影响归因于制造过程中产生的二恶英和其他污染物。在该鱼中，两种等级的化合物均对峰值和总CL产生显著的剂量依赖性抑制。然而，在相同的亚致死浓度下，两种五氯苯酚等级对CL反应幅度的影响之间几乎没有显著差异，尽管T-PCP确实比A-PCP更显著地降低了白细胞CL的相对水平。由于ROI在抗微生物血细胞介导机制中的可能作用，CL活性降低被解释为外源性诱导的免疫抑制的指示。
Chemiluminescence (CL) was used to assay effects of pentachlorophenol (PCP) on the production of reactive oxygen intermediates (ROIs) generated by leukocytes from an estuarine teleost,Fundulus heteroclitus. Two parameters were measured during the phagocytically-induced respiratory burst: peak CL (maximal value) and total CL (temporal summation of induced values). Phagocytes obtained from the pronephros were incubated in the presence of sublethal doses of analytical (A-) or technical (T-) grades of PCP. Technical PCP has been reported to be more highly immunosuppressive than A-PCP in mammals; this effect was attributed to dioxins and other contaminants produced during manufacture. In this fish, both grades of the compound produced significant dose-dependent inhibition of peak and total CL. However, there were few significant differences between the effects of the two PCP grades on the magnitude of the CL responses at identical, sublethal concentrations, although T-PCP did produce a more marked reduction in the relative level of leukocytic CL as a function of dose than did A-PCP. Because of the probable roles of ROIs in antimicrobial blood cell-mediated mechanisms, reduced CL activity was interpreted as an indication of xenobiotic-induced immunosuppression.