STRUCTURAL AND QUANTITATIVE STUDIES ON THE NORMAL C3H AND LURCHER MUTANT MOUSE
STRUCTURAL AND QUANTITATIVE STUDIES ON THE NORMAL C3H AND LURCHER MUTANT MOUSE
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DOI：
10.1098/rstb.1979.0055
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发表时间：
1979-01-01
影响因子：
6.3
通讯作者：
BISCOE, TJ
中科院分区：
文献类型：
--
作者：
CADDY, KWT;BISCOE, TJ
用光学显微镜和电子显微镜对杂合子Lurcher突变小鼠的小脑、小脑深核和下橄榄核与正常仔鼠的相同结构进行了比较。研究的小鼠年龄从4-730天不等。成年Lurcher小鼠的小脑比正常小得多。未见浦肯野细胞，内颗粒细胞层厚度和密度降低。对年轻动物的检查表明，浦肯野细胞存在，并经历了退化。在成体Lurcher突变体中，颗粒细胞数量减少，并在发育过程中出现退行性变化。高尔基体细胞和星状细胞相对正常，可见一些篮状细胞。Lurcher突变体的下橄榄核与正常小鼠一样广泛。正常小鼠小脑深部核团不易分为外侧核、间位核和内侧核。在Lurcher突变体中，神经元的大小与正常小鼠相似，但它们比正常小鼠更紧密地聚集在一起。在Lurcher突变体中，与正常成人一样，神经元胞体被突触覆盖，而不是神经胶质细胞。对细胞总数的估计是为了获得结构变化发展的时间进程的证据，并详细比较正常小鼠和Lurcher突变体在浦肯野细胞、颗粒细胞、橄榄神经元和小脑深核神经元方面的差异。将Lurcher突变体中的损伤与其他小鼠小脑突变体中发现的损伤进行比较，并与实验中诱发的小脑损伤进行比较。对于Lurcher突变体，原发病变可能发生在浦肯野细胞。
The cerebellum, the deep cerebellar nuclei and the inferior olivary nuclus of the heterozygote Lurcher mutant mouse were compared with the same structures in normal littermates using light microscopy and EM. The mice studied were aged from 4-730 days. In the adult Lurcher mouse the cerebellum is much smaller than is normal. There are no Purkinje cells and the internal granule cell layer is reduced in thickness and density. Examination of younger animals shows that Purkinje cells are present and that they undergo degeneration. Granule cells in the adult Lurcher mutant are reduced in number and during the developmental period degenerative changes are seen. Golgi cells and stellate cells are relatively normal and some cells, identified as basket cells, are seen. The inferior olivary nucleus is as extensive in the Lurcher mutant as in the normal mouse. The deep cerebellar nuclei in the normal mouse cannot be separated easily into their 3 subdivisions.sbd.lateral, interpositus and medial. In the Lurcher mutant the neurons are of similar size to those of the normal mouse but they are crowded more closely together than in normal. In the Lurcher mutant as in the normal adult the neuronal cell bodies are covered with synapses and not with glial cells. Estimates of total cells numbers were made to obtain evidence about the time course of the development of the changes in structure and to make a detailed comparison between the normal mouse and the Lurcher mutant with respect to Purkinje cells, granule cells, olive neurons and deep cerebellar nuclei neurons. The lesion in the Lurcher mutant is compared with that found in the other mouse cerebellar mutants and with experimentally evoked lesions of the cerebellum. For the Lurcher mutant, the primary lesion may arise in the Purkinje cells.