Gene organization and sequence determination of the two botulinum neurotoxin gene clusters in Clostridium botulinum type A(B) strain NCTC 2916
Gene organization and sequence determination of the two botulinum neurotoxin gene clusters in Clostridium botulinum type A(B) strain NCTC 2916
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DOI：
10.1007/s002849900299
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发表时间：
1998-04-01
影响因子：
2.6
通讯作者：
East, AK
中科院分区：
文献类型：
--
作者：
Jovita, MR;Collins, MD;East, AK
研究了肉毒梭菌NCTC 2916株A型和B型肉毒毒素基因簇的基因结构和核苷酸序列。目的是阐明C.具有未表达BoNT/B基因的A型肉毒杆菌菌株。BoNT/A基因簇包括编码BoNT、NTNH和P-47的一部分的基因（该蛋白的基因在C.肉毒杆菌E型和F型）。与沉默BoNT/B基因杂交的是编码NTNH、P-21和HA-33的基因。NTNH的测序分析揭示了B型和A型基因簇中存在471个相同的氨基酸。该基因组织与亨德森等人（FEMS Microbiol. Lett. 140. 151-158）。在A型（B）菌株NCTC 2916中，神经毒素基因属于基因簇内的BoNT/A1型，该基因簇与BoNT/A2型菌株中发现的基因簇具有相同的组织结构;这些观察结果在确定BoNT基因簇的起源方面可能具有重要意义。
The gene organization and nucleotide sequence of the type A and B BoNT-gene clusters in Clostridium botulinum strain NCTC 2916 were studied. The aim was to clarify the organization of genes within C. botulinum type A strains possessing an unexpressed BoNT/B gene. The BoNT/A-gene cluster includes genes encoding BoNT, NTNH and a part of P-47 (the gene for this protein was reported in strains of C. botulinum types E and F). Clustered with the silent BoNT/B gene were genes encoding NTNH, P-21 and HA-33. Sequencing analysis of the: NTNHs revealed the presence of 471 amino acids identical in the type B and A gene clusters. This gene organization contrasts markedly with the purported organization in strain NCTC 2916 described by Henderson et al. (FEMS Microbiol. Lett. 140. 151-158). In type A(B) strain NCTC 2916, the neurotoxin gene is of type BoNT/A1 within a gene cluster that has identical organization to that found in BoNT/A2 type strains; these observations may be significant in establishing the origin of the BoNT-gene cluster.