TESTICULAR TISSUE-SPECIFIC EXPRESSION OF THE P53 SUPPRESSOR GENE
TESTICULAR TISSUE-SPECIFIC EXPRESSION OF THE P53 SUPPRESSOR GENE
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DOI：
10.1006/dbio.1993.1062
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发表时间：
1993-03-01
影响因子：
2.7
通讯作者：
ROTTER, V
中科院分区：
文献类型：
--
作者：
ALMON, E;GOLDFINGER, N;ROTTER, V
杂交转基因方法适用于研究p53抑制基因参与的生理途径。p53启动子-CAT转基因小鼠的CAT酶活性主要在睾丸中表达，原位杂交结果表明，转基因和内源性p53的表达符合精子发生的典型波型和周期模式。在睾丸中表达的p53启动子-CAT转基因小鼠降低了与小鼠或人转基因拷贝数相关的内源性p53 mRNA水平。p53基因的空间和周期性表达仅限于曲细精管的初级精母细胞，提示p53基因可能在体内精子发生的减数分裂过程中起作用。
The hybrid transgene approach was adapted to study the physiological pathway(s) in which the p53 suppressor gene is involved. p53 promoter-CAT transgenic mice were found to express enzymatic CAT activity predominantly in the testes.In situhybridization indicated that expression of the transgene as well as the endogenous p53 agreed with the typical wave and cycle patterns of spermatogenesis. p53 promoter-CAT transgenic mice expressed in the testes reduced levels of endogenous p53 mRNA that correlated with the copy number of the mouse or human transgene. The spatial and cyclical expression of the p53 gene which is confined to the primary spermatocytes in the seminiferous tubuli suggested that p53 may play a role in the meiotic process of spermatogenesisin vivo.