Contrasting impacts of ocean acidification and warming on the molecular responses of CO(2)-resilient oysters.
Contrasting impacts of ocean acidification and warming on the molecular responses of CO(2)-resilient oysters.
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DOI：
10.1186/s12864-017-3818-z
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发表时间：
2017-06-02
期刊：
影响因子：
4.4
通讯作者：
Raftos DA
中科院分区：
文献类型：
--
作者：
Goncalves P;Thompson EL;Raftos DA
这项研究的特点改变了分子过程中的CO2浓度升高和温度升高的牡蛎。海洋生物对与气候变化有关的环境压力的复原力的差异将对全世界沿海生态系统的可持续性产生重大影响。一些证据表明，气候变化的适应能力在一个物种内的种群之间可能存在差异。B2牡蛎代表了一种独特的遗传资源，因为与来自同一物种的非选择性牡蛎（Saccostrea glomerata）相比，它们能够更好地承受生理水平上CO2升高的影响。在这里，我们使用鳃组织的蛋白质组学和转录组学分析来评估B2牡蛎对CO2浓度升高的差异反应是否也延伸到温度升高。B2牡蛎响应CO2浓度升高或温度升高的蛋白质浓度和基因表达的显着和独特的影响是显而易见的。这两种压力的组合也改变了牡蛎鳃蛋白质组和基因表达。然而，CO2和温度升高的影响不是加和或协同的，而可能是拮抗的。这些数据表明，同时暴露于不久的将来预计的海洋pH值和温度的CO2弹性牡蛎的分子过程中的复杂变化，以防止应力引起的细胞损伤。B2牡蛎的组合压力的差异反应也表明，热应力的加入可能会损害这些牡蛎的弹性，降低pH值。总体而言，这项研究揭示了细胞内的机制，可能使海洋钙化忍受紧急，不利的海水条件下，气候变化。本文的在线版本（doi：10.1186/s12864-017-3818-z）包含补充材料，可供授权用户使用。
This study characterises the molecular processes altered by both elevated CO2 and increasing temperature in oysters. Differences in resilience of marine organisms against the environmental stressors associated with climate change will have significant implications for the sustainability of coastal ecosystems worldwide. Some evidence suggests that climate change resilience can differ between populations within a species. B2 oysters represent a unique genetic resource because of their capacity to better withstand the impacts of elevated CO2 at the physiological level, compared to non-selected oysters from the same species (Saccostrea glomerata). Here, we used proteomic and transcriptomic analysis of gill tissue to evaluate whether the differential response of B2 oysters to elevated CO2 also extends to increased temperature. Substantial and distinctive effects on protein concentrations and gene expression were evident among B2 oysters responding to elevated CO2 or elevated temperature. The combination of both stressors also altered oyster gill proteomes and gene expression. However, the impacts of elevated CO2 and temperature were not additive or synergistic, and may be antagonistic. The data suggest that the simultaneous exposure of CO2-resilient oysters to near-future projected ocean pH and temperature results in complex changes in molecular processes in order to prevent stress-induced cellular damage. The differential response of B2 oysters to the combined stressors also indicates that the addition of thermal stress may impair the resilience of these oysters to decreased pH. Overall, this study reveals the intracellular mechanisms that might enable marine calcifiers to endure the emergent, adverse seawater conditions resulting from climate change. The online version of this article (doi:10.1186/s12864-017-3818-z) contains supplementary material, which is available to authorized users.