Nitrogen and hydrophosphate affects glycolipids composition in microalgae.
Nitrogen and hydrophosphate affects glycolipids composition in microalgae.
复制标题
氮和磷酸氢盐影响微藻中的糖脂组成
DOI：
10.1038/srep30145
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发表时间：
2016-07-21
影响因子：
4.6
通讯作者：
Miao X
中科院分区：
文献类型：
--
作者：
Wang X;Shen Z;Miao X
糖脂因其在化妆品、医药、食品、机械制造等行业的广泛应用而受到越来越多的关注。微藻是一种竞争性的代谢物质积累生物。然而，利用微藻生产糖脂目前还不多见。本研究以蛋白核小球藻（Chlorella pyrenoidosa）和集球藻（Synechococcus sp.在不同的硝酸盐和磷酸氢盐水平下进行了研究。糖脂含量最高的是C.聚球藻属（Synechococcussp.）在无硝酸盐条件下，C.聚球藻属（Synechococcussp.）分别为0.01和0 g L-1磷酸氢盐。两种微藻的糖脂产率均达到10.59 mg L−1d−1以上。硝酸盐缺乏导致两种微藻的MGDG、DGDG和SQDG至少增加8.5%，而磷酸氢盐缺乏导致两种微藻的DGDG增加超过21.2%，SQDG增加超过48.4%，MGDG减少超过22.2%。同时限制硝酸盐和磷酸氢盐可以进一步改善糖脂积累，C的糖脂积累量超过25%。蛋白核菌（Pyrenoidosa）和集球藻（Synechococcussp.这些结果表明，氮磷限制或饥饿是提高微藻糖脂积累的有效途径。
Glycolipids had received increasing attention because of their uses in various industries like cosmetics, pharmaceuticals, food and machinery manufacture. Microalgae were competitive organisms to accumulate metabolic substance. However, using microalgae to produce glycolipid was rare at present. In this study, glycolipid content ofChlorella pyrenoidosaandSynechococcussp. under different nitrate and hydrophosphate levels were investigated. The highest glycolipid contents of 24.61% forC. pyrenoidosaand 15.37% forSynechococcussp. were obtained at nitrate absence, which were 17.19% forC. pyrenoidosaand 10.99% forSynechococcussp. at 0.01 and 0 g L−1hydrophosphate, respectively. Glycolipid productivities of two microalgae could reach at more than 10.59 mg L−1d−1. Nitrate absence induced at least 8.5% increase in MGDG, DGDG and SQDG, while hydrophosphate absence resulted in over 21.2% increase in DGDG and over 48.4% increase in SQDG and more than 22.2% decrease in MGDG in two microalgae. Simultaneous nitrate and hydrophosphate limitation could make further improvement of glycolipid accumulation, which was more than 25% forC. pyrenoidosaand 21% forSynechococcussp. These results suggest that nitrogen and phosphorus limitation or starvation should be an efficient way to improve microalgal glycolipid accumulation.