Antimutagenic activity of spearmint.
Antimutagenic activity of spearmint.
复制标题
留兰香的抗突变活性。
DOI：
10.1002/em.20063
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
Dashwood,RoderickH
中科院分区：
文献类型：
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作者：
Yu,Tian-Wei;Xu,Meirong;Dashwood,RoderickH
采用沙门氏菌法研究了一种常用的食品调味剂——绿薄荷的抗诱变活性。将绿薄荷叶以高达5% （w/v）的浓度在热水中冲泡5分钟，并使用鼠伤寒沙门氏菌TA98菌株对直接作用诱变剂4 -硝基- 1,2 -苯二胺（NPD）和2 -羟氨基- 3 -甲基- 3H -咪唑[4,5 - f]喹啉（N - OH - IQ）进行测试。无毒浓度的绿薄荷提取物以浓度依赖的方式抑制n - OH - IQ的诱变活性，但对NPD没有影响。这些实验集中在通常作为凉茶食用的水提取物上，但绿薄荷的氯仿和甲醇提取物也具有抗n - OH - IQ的抗诱变活性。绿薄荷水提物在大鼠肝脏S9存在下抑制母体化合物2‐氨基‐3‐甲基‐3H‐咪唑[4,5‐f]喹啉（IQ）的诱变活性；然而，对IQ的50%抑制浓度（IC50）比在n - OH - IQ - S9条件下的实验高约10倍。根据乙氧基间苯二酚-去乙基酶和甲氧基间苯二酚-去甲基化酶的体外实验，在与沙门氏菌实验相似的浓度下，薄荷提取物抑制了两种在IQ代谢激活中起作用的主要酶，即细胞色素P4501A1和1A2。在体内，在IQ治疗2周之前、期间和之后，大鼠被给予留兰叶水提取物（2%;w/v）作为唯一的饮水来源；8周时结肠隐窝异常灶明显受到抑制（P< 0.05）。总的来说，这些发现表明，薄荷茶通过抑制致癌物质的激活和对活性代谢物的直接作用，可以防止智商和其他杂环胺的侵害。环绕。摩尔,诱变剂。, 2004年。©2004 Wiley‐Liss, Inc。
The antimutagenic activity of spearmint (Mentha spicata), a popular food flavoring agent, was studied in the Salmonella assay. Spearmint leaves were brewed in hot water for 5 min at concentrations up to 5% (w/v), and the water extracts were tested against the direct‐acting mutagens 4‐nitro‐1,2‐phenylenediamine (NPD) and 2‐hydroxyamino‐3‐methyl‐3H‐imidazo[4,5‐f]quinoline (N‐OH‐IQ) usingSalmonella typhimuriumstrain TA98. Nontoxic concentrations of spearmint extract inhibited the mutagenic activity ofN‐OH‐IQ in a concentration‐dependent fashion, but had no effect against NPD. These experiments by design focused on the water extract consumed commonly as an herbal tea, but chloroform and methanol extracts of spearmint also possessed antimutagenic activity againstN‐OH‐IQ. Water extract of spearmint inhibited the mutagenic activity of the parent compound, 2‐amino‐3‐methyl‐3H‐imidazo[4,5‐f]quinoline (IQ), in the presence of rat liver S9; however, the concentration for 50% inhibition (IC50) against IQ was approximately 10‐fold higher than in assays withN‐OH‐IQ minus S9. At concentrations similar to those used in the Salmonella assays, spearmint extract inhibited two of the major enzymes that play a role in the metabolic activation of IQ, namely, cytochromes P4501A1 and 1A2, based on ethoxyresorufinO‐deethylase and methoxyresorufinO‐demethylase assays in vitro. In vivo, rats were given spearmint water extract (2%; w/v) as the sole source of drinking fluid before, during, and after 2‐week treatment with IQ; colonic aberrant crypt foci were inhibited significantly at 8 weeks (P< 0.05, compared with rats given IQ alone). Collectively, these findings suggest that spearmint tea protects against IQ and possibly other heterocyclic amines through inhibition of carcinogen activation and via direct effects on the activated metabolite(s). Environ. Mol. Mutagen., 2004. © 2004 Wiley‐Liss, Inc.