Gene expression changes in normal human skin fibroblasts induced by HZE-particle radiation
Gene expression changes in normal human skin fibroblasts induced by HZE-particle radiation
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DOI：
10.1667/rr3350.1
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发表时间：
2005-10-01
影响因子：
3.4
通讯作者：
Chen, DJ
中科院分区：
文献类型：
--
作者：
Ding, LH;Shingyoji, M;Chen, DJ
研究表明，辐射暴露会影响哺乳动物细胞中的全球基因表达。然而，人们对HZE颗粒对基因表达的影响知之甚少。为了研究这些效应，用不同能量和LET的HZE粒子照射人皮肤成纤维细胞。从这些实验中获得的数据表明，基因表达的变化取决于放射源的能量。能量最高的粒子，即铁，在基因数量和变化幅度方面引起的表达变化最大。经HZE粒子照射后，CDKN1A/p21、MDM2、TNFRSF6/fas、增殖细胞核抗原和RAD52等基因的表达发生了显著变化。与X射线不同，HZE粒子将细胞暴露在两种类型的辐射下：初级离子和8射线。我们假设六种射线的生物效应，即二次电子发射，应该类似于X射线的效应。为了探索这一想法，我们比较了HZE粒子和X射线照射后细胞之间的基因表达变化。这一结果支持了我们的假设，因为在两次辐射后通常发生变化的基因数量随着粒子能量的增加而增加。(C)2005年，由辐射研究学会提供。
Studies have shown that radiation exposure affects global gene expression in mammalian cells. However, little is known about the effects of HZE particles on gene expression. To study these effects, human skin fibroblasts were irradiated with HZE particles of different energies and LETS. The data obtained from these experiments indicate that changes in gene expression are dependent on the energy of the radiation source. Particles with the highest energy, i.e. iron, induced the biggest expression changes in terms of numbers of genes and magnitudes of changes. Many genes were found to undergo significant expression changes after HZE-particle irradiation, including CDKN1A/p21, MDM2, TNFRSF6/fas, PCNA and RAD52. Unlike X rays, HZE particles expose cells to two types of radiation: primary ions and 8 rays. We hypothesized that the biological effects of 6 rays, which are secondary electron emissions, should resemble the effects of X rays. To explore this idea, gene expression changes between cells that had been irradiated with HZE particles and X rays were compared. The results support our hypothesis since the number of genes that commonly changed after exposure to both radiations increased as a function of particle energy. (c) 2005 by Radiation Research Society.