fringe and Notch specify polar cell fate during Drosophila oogenesis.
fringe and Notch specify polar cell fate during Drosophila oogenesis.
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发表时间：
2001-06
期刊：
影响因子：
4.6
通讯作者：
Muriel Grammont;Kenneth D. Irvine
中科院分区：
文献类型：
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作者：
Muriel Grammont;Kenneth D. Irvine
条纹编码糖基转移酶，调节Notch受体被其配体激活的能力。我们描述了在果蝇卵发生的早期阶段边缘功能的研究。边缘次形等位基因突变的动物含有不正确数量的生殖系细胞的卵泡，这些卵泡被异常长且无组织的茎分开。对条带空等位基因突变体细胞克隆的分析，将卵泡形成对条带的要求定位于极性细胞，并证明了极性细胞的命运需要条带。Notch突变的细胞克隆也缺乏极性细胞，卵泡形成中对Notch的需求似乎映射到极性细胞。异位表达条纹或激活形式的Notch可以产生额外的极细胞。我们的研究结果表明，在卵子发生早期，边缘在定位Notch激活中起着关键作用，并在将种系囊肿分离为单个卵泡中建立了极地细胞的功能。
fringe encodes a glycosyltransferase that modulates the ability of the Notch receptor to be activated by its ligands. We describe studies of fringe function during early stages of Drosophila oogenesis. Animals mutant for hypomorphic alleles of fringe contain follicles with an incorrect number of germline cells, which are separated by abnormally long and disorganized stalks. Analysis of clones of somatic cells mutant for a null allele of fringe localizes the requirement for fringe in follicle formation to the polar cells, and demonstrates that fringe is required for polar cell fate. Clones of cells mutant for Notch also lack polar cells and the requirement for Notch in follicle formation appears to map to the polar cells. Ectopic expression of fringe or of an activated form of Notch can generate an extra polar cell. Our results indicate that fringe plays a key role in positioning Notch activation during early oogenesis, and establish a function for the polar cells in separating germline cysts into individual follicles.