Interactions between human osteoarthritic chondrocytes and synovial fibroblasts in co-culture.
Interactions between human osteoarthritic chondrocytes and synovial fibroblasts in co-culture.
复制标题
共培养中人骨关节炎软骨细胞和滑膜成纤维细胞之间的相互作用。
DOI：
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发表时间：
2001
影响因子：
3.7
通讯作者：
W. Puhl
中科院分区：
文献类型：
--
作者：
Klaus Huch;J. Stöve;K. Günther;W. Puhl
目的
为了模拟体内关节情况并允许细胞相互作用，建立了来自单个关节的人骨关节炎软骨细胞和滑膜成纤维细胞的共培养系统，并在有或没有IL-1 β刺激的情况下进行表征。
方法
培养设置包括单独藻酸盐中的软骨细胞、单独单层中的滑膜成纤维细胞以及两者的共培养物。通过35S-掺入法测定蛋白聚糖（PG）的合成，通过二甲基亚甲蓝测定法测定PG含量，通过荧光测定法测定DNA含量，通过ELISA测定伊兰素-E2和IL-1 β的释放。
结果
在共培养中，与对照相比，在IL-1 β（1 ng/ml）存在下，软骨细胞的PG合成显著减少。对于对照和IL-1 β处理的共培养物，软骨细胞培养物的PG含量降低。与单细胞培养的细胞相比，共培养的滑膜成纤维细胞的PG合成或含量没有显着变化。在共培养物中，PG释放到培养基中相对较高。IL-1 β显著降低了共培养物中软骨细胞的增殖率，并略微增加了软骨素-E2的释放。
结论
来自单个人关节的骨关节炎软骨细胞和滑膜成纤维细胞的共培养允许两个实体之间的相互作用，并且可以提供有用的工具来研究与单细胞培养物相比在更类似于体内的条件下介质或新药的作用。
OBJECTIVE
To imitate the in vivo joint situation and to allow cell interactions, a co-culture system of human osteoarthritic chondrocytes and synovial fibroblasts from a single joint was established and characterized with or without stimulation by IL-1 beta.
METHODS
Culture settings included chondrocytes in alginate alone, synovial fibroblasts in monolayer alone and a co-culture of both. Proteoglycan (PG) synthesis was measured by 35S-incorporation, PG content by a dimethylmethylene blue assay, DNA content by a fluorometric assay, and prostaglandin-E2 and IL-1 beta release by ELISA.
RESULTS
In co-culture PG synthesis by chondrocytes was significantly reduced in the presence of IL-1 beta (1 ng/ml) compared to controls. PG content of chondrocyte cultures was reduced for controls and IL-1 beta treated co-cultures. Synovial fibroblasts in co-culture did not show significant change of PG synthesis or content when compared to cells in mono-cell culture. PG release into the medium was relatively high in co-cultures. IL-1 beta significantly decreased the proliferation rate of chondrocytes in co-cultures and slightly increased prostaglandin-E2 release.
CONCLUSIONS
Co-culturing of osteoarthritic chondrocytes and synovial fibroblasts from a single human joint allows interactions between both entities and may offer a useful tool to study the effects of mediators or new drugs under more in vivo like conditions compared to mono-cell cultures.
影响因子：
2.9
作者：
KIM, YJ;SAH, RLY;GRODZINSKY, AJ
通讯作者：
GRODZINSKY, AJ
影响因子：
2.9
作者：
GUO, JF;JOURDIAN, GW;MACCALLUM, DK
通讯作者：
MACCALLUM, DK
影响因子：
4
作者：
Hans J. Häuselmann;Russell J. Fernandes;Su S. Mok;Thomas M. Schmid;Joel A. Block;M. Aydelotte;Klaus E. Kuettner;E. Thonar
通讯作者：
Hans J. Häuselmann;Russell J. Fernandes;Su S. Mok;Thomas M. Schmid;Joel A. Block;M. Aydelotte;Klaus E. Kuettner;E. Thonar