A nano-view of West Nile virus-induced cellular changes during infection.
A nano-view of West Nile virus-induced cellular changes during infection.
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DOI：
10.1186/1477-3155-2-6
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发表时间：
2004-06-29
影响因子：
10.2
通讯作者：
Ng, Mah-Lee
中科院分区：
文献类型：
--
作者：
Lee, Jason WM;Ng, Mah-Lee
背景：病毒的显微成像及其与宿主细胞的相互作用和对宿主细胞的影响往往受到显微镜分辨率的限制或样品制备过程的侵入性的限制。也很难有一种技术允许在同一电池上同时对表面和亚表面进行成像。这阻碍了阐明病毒与宿主相互作用的努力。原子力显微镜(AFM)是物理科学中常用的显微镜技术，现已成为补充现有光学显微镜、共聚焦显微镜和电子显微镜用于生物学应用的一种良好的相关形式。结果：在本研究中，使用了西尼罗河(Sarafend)病毒感染的Vero细胞模型。原子力显微镜被发现在产生高分辨率的病毒宿主事件的图像方面很有用，只需要最少的样本处理需求。AFM能够在受感染的细胞表面成像西尼罗河(萨拉芬德)病毒的萌芽。伴随着西尼罗河病毒的复制，丝足的增殖和肌动蛋白细丝簇的增厚。结论：原子力显微镜可用于病毒-宿主相互作用的研究。该技术在感染阶段提供了关于病毒和宿主细胞的形态信息。
BACKGROUND: Microscopic imaging of viruses and their interactions with and effects on host cells are frequently held back by limitations of the microscope's resolution or the invasive nature of the sample preparation procedures. It is also difficult to have a technique that would allow simultaneous imaging of both surface and sub-surface on the same cell. This has hampered endeavours to elucidate virus-host interactions. Atomic Force Microscopy (AFM), which is commonly used in the physical sciences, is now becoming a good correlative form of microscopy used to complement existing optical, confocal and electron microscopy for biological applications RESULTS: In this study, the West Nile (Sarafend) virus-infected Vero cell model was used. The atomic force microscope was found to be useful in producing high resolution images of virus-host events with minimal sample processing requirements. The AFM was able to image the budding of the West Nile (Sarafend) virus at the infected cell surface. Proliferation of the filopodia and thickening of clusters of actin filaments accompanied West Nile virus replication. CONCLUSIONS: The study shows that the AFM is useful for virus-host interaction studies. The technique provides morphological information on both the virus and the host cell during the infection stages.