Hollow fiber post-column reactor for liquid chromatography
Hollow fiber post-column reactor for liquid chromatography
复制标题
液相色谱用中空纤维柱后反应器
DOI：
10.1021/ac00280a044
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
D. Peterson
中科院分区：
文献类型：
--
作者：
James C. Davis;D. Peterson
先生：柱后衍生化技术用于液相色谱柱洗脱物的强化检测，已引起人们的广泛关注。Frei和Scholten（1）已经回顾了反应检测器的许多理论和技术方面，包括与其使用相关的问题，而Takata和Muto（2）首先讨论了用于增强电化学检测等用途的反应检测器。据报道，中空纤维膜可用于离子色谱的未填充和填充中空纤维抑制器中的柱后反应（3，4）。良好的柱后衍生化要求可简化为三个关键部分：（a）试剂引入，（B）延迟或反应/混合回路，以及（c）适当的检测装置。（B）和（c）两者基本上由所选择的反应时间/温度和化学性质固定。a部分，反应堆本身，允许更大的灵活性。例如，对于试剂混合，诸如空气分段、将试剂泵送到混合装置中以及试剂颗粒的填充床的技术都已被使用。不幸的是，这些方法中的每一种都有其缺点。空气分割和混合装置需要额外的泵，并导致带扩散和稀释，
Sir: A great deal of attention has been focused on po-stcolumn derivatization for enhanced detection of species eluting from liquid chromatographycolumns. Frei and Scholten (1) have reviewed many of the theoretical and technical aspects of reaction detectors, including the problems associated with their use, while Takata and Muto (2) first discussed reaction detectorsfor uses such as enhanced elec-trochemical detection. Hollow fiber membranes have been reported for postcolumn reaction in both unpacked and packed hollow fiber suppressors for ion chromatography (3, 4).The requirements for good postcolumn derivatization can be reduced tothree key components:(a) reagent introduction,(b) delay or reaction/mixing loops, and (c) properdetection devices. Both (b) and (c) are essentially fixed by the reaction time/temperature and chemistry chosen. Part a, the reactor itself, allows more flexibility. For example, with reagent mixing, techniquessuch as air segmentation, pumping reagent into a mixing device, and packed beds of reagent granules have all been used. Unfortunately, each of these methods has its drawbacks. Air segmentation and mixing devices require an additional pump and lead to band spreading and dilution,