Differentiation of HL-60 cells: cell volume and cell cycle changes.
Differentiation of HL-60 cells: cell volume and cell cycle changes.
复制标题
HL-60细胞的分化：细胞体积和细胞周期的变化。
DOI：
--
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
B. J. Price
中科院分区：
文献类型：
--
作者：
R. Zucker;K. Whittington;B. J. Price
HL-60早幼粒细胞白血病细胞可以通过添加二甲亚砜、丁酸或视黄酸分化为更成熟的骨髓细胞，并且可以通过添加佛波酯12-0-十四烷酰佛波醇-13-乙酸酯(TPA)分化为巨噬细胞。添加诱导剂后，HL-60 细胞体积显示每日减少，而细胞数量以与未处理的对照细胞相似的速度增加。流式细胞术测量显示第 1 天后 G1 细胞增加，S 细胞减少。由于世代时间恒定，数据表明在分化过程中不同细胞周期阶段所花费的时间长度发生了变化。将TPA添加到HL-60细胞后3小时内，G1细胞发生选择性粘附。从烧瓶底部回收较小尺寸的细胞，从上清液回收较大尺寸的细胞。流式细胞术分析显示从上清液和烧瓶底部获得的细胞中存在 G1 和 S 块。 TPA 孵育 1 天后，G1 和 G2 细胞优先粘附，非粘附细胞主要处于 S 和 G2 细胞周期阶段并经历细胞周期遍历。
HL-60 promyelocytic leukemic cells can differentiate into more mature myeloid cells with the addition of dimethylsulfoxide, butyric acid or retinoic acid and can differentiate into macrophages with the addition of phorbol ester 12-0-tetradecanoylphorbol-13-acetate (TPA). After the addition of an inducer, the HL-60 cell volume shows a daily decrease while the cell number increases at a rate similar to the untreated control cells. Flow cytometry measurements show an increase in G1 cells and a decrease in S cells after day 1. Since the generation time is constant, the data suggest that the length of time spent in the different cell cycle stages has changed during differentiation. Within 3 hours after the addition of TPA to HL-60 cells, selective adhesion of G1 cells occurs. Smaller sized cells are recovered from the flask bottom and larger sized cells are recovered from the supernate. Flow cytometric analysis reveals a G1 and S block in cells obtained from both the supernatant and from the flask bottom. After 1 day of TPA incubation, there is preferential adhesion of G1 and G2 cells with the nonadherent cells being primarily in the S and G2 cell cycle stages and undergoing a cell cycle traverse.