A correlation analysis of Light Microscopy and X-ray MicroCT imaging methods applied to archaeological plant remains' morphological attributes visualization
A correlation analysis of Light Microscopy and X-ray MicroCT imaging methods applied to archaeological plant remains' morphological attributes visualization
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DOI：
10.1038/s41598-020-71726-z
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发表时间：
2020-09-15
影响因子：
4.6
通讯作者：
Neves, Eduardo G.
中科院分区：
文献类型：
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作者：
Calo, Cristina Marilin;Rizzutto, Marcia A.;Neves, Eduardo G.
在这项工作中，通过两种不同的成像方法，分析了在巴西朗多尼亚考古遗址Monte Castelo发现的核果内部形态的几个属性。目的是探讨通过高分辨率光学显微镜和x射线微计算机断层扫描（x射线微ct）方法获得的图像的可视化和分析特性的异同。两者都提供了研究样本的三层果皮（外、中、内果皮）的数据，通过细胞分化、血管化、细胞内容物、厚壁组织细胞和分泌腔的存在来定义。然而，可以识别出通过每种方法获得的信息之间的一系列差异。这些变化与某些特征的轮廓和精细细节的定义、空间分布、尺寸属性、光学性质和材料保存有关。从两种成像方法获得的结果是互补的，有助于对植物遗骸进行更详尽的形态学研究。当需要保存样品时，相衬模式下的x射线微ct是一种合适的无损分析技术。
In this work, several attributes of the internal morphology of drupaceous fruits found in the archaeological site Monte Castelo (Rondonia, Brazil) are analyzed by means of two different imaging methods. The aim is to explore similarities and differences in the visualization and analytical properties of the images obtained via High Resolution Light Microscopy and X-ray micro-computed tomography (X-ray MicroCT) methods. Both provide data about the three-layered pericarp (exo-, meso- and endocarp) of the studied exemplars, defined by cell differentiation, vascularisation, cellular contents, presence of sclerenchyma cells and secretory cavities. However, it is possible to identify a series of differences between the information that can be obtained through each of the methods. These variations are related to the definition of contours and fine details of some characteristics, their spatial distribution, size attributes, optical properties and material preservation. The results obtained from both imaging methods are complementary, contributing to a more exhaustive morphological study of the plant remains. X-ray MicroCT in phase-contrast mode represents a suitable non-destructive analytic technique when sample preservation is required.